Human iPSCs-derived mesenchymal stem cells promote skin regeneration and burn wound healing.

IF 6.5 1区医学 Q1 CELL & TISSUE ENGINEERING

npj Regenerative Medicine Pub Date : 2025-08-30 DOI:10.1038/s41536-025-00427-w

Mahmoud Farahat, Sophie Brosset, Yufei Chen, Ayesha Aijaz, Graham Rix, Bhavishya Challagundla, Margarita Elloso, Maria Fernanda Hutter, Ian M Rogers, Marc G Jeschke

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Abstract

The key to surviving severe burns is rapid burn wound excision and closure, yet extensive wounds often surpass natural healing capacity. Alternative treatments, such as synthetic skin substitutes, have not emerged as a standard, optimal solution. Stem cell therapies, especially using allogenic sources, show promise in enhancing wound repair. Induced mesenchymal stem cells (iMSCs) have demonstrated vast possibilities to overcome traditional stem cell therapy limitations. This study utilized Cord tissue-derived iMSCs (CT-iMSCs) incorporated into well-established epidermal-dermal substitutes Integra® Dermal Regeneration Template (DRT) at 5000-20,000 cells/cm² in a porcine full-thickness burn model to test their regenerative capabilities. We evaluated healing outcomes, inflammation, neovascularization, collagen levels, and fibrosis markers. Wounds treated with CT-iMSCs showed notable improvements, including faster wound healing, better epithelialization, and marked improvements in healing markers compared to controls. These data support the potential of iMSCs as an ideal cell source for autologous skin regeneration.

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人类ipscs衍生的间充质干细胞促进皮肤再生和烧伤创面愈合。

严重烧伤存活的关键是迅速切除和闭合烧伤创面，但大面积创面往往超过自然愈合能力。替代疗法，如合成皮肤替代品，尚未成为标准的最佳解决方案。干细胞治疗，特别是使用同种异体来源，在增强伤口修复方面显示出希望。诱导间充质干细胞（iMSCs）已经证明了克服传统干细胞治疗局限性的巨大可能性。本研究利用脐带组织来源的iMSCs （CT-iMSCs）与成熟的表皮真皮替代品Integra®真皮再生模板（DRT）结合，以5000-20,000细胞/cm2的速度在猪全层烧伤模型中测试其再生能力。我们评估了愈合结果、炎症、新生血管、胶原蛋白水平和纤维化标志物。与对照组相比，用CT-iMSCs治疗的伤口表现出显著的改善，包括伤口愈合更快，上皮化更好，愈合标志物明显改善。这些数据支持了iMSCs作为自体皮肤再生的理想细胞来源的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

npj Regenerative Medicine Engineering-Biomedical Engineering

CiteScore

10.00

自引率

1.40%

发文量

审稿时长

12 weeks

期刊介绍： Regenerative Medicine, an innovative online-only journal, aims to advance research in the field of repairing and regenerating damaged tissues and organs within the human body. As a part of the prestigious Nature Partner Journals series and in partnership with ARMI, this high-quality, open access journal serves as a platform for scientists to explore effective therapies that harness the body's natural regenerative capabilities. With a focus on understanding the fundamental mechanisms of tissue damage and regeneration, npj Regenerative Medicine actively encourages studies that bridge the gap between basic research and clinical tissue repair strategies.