Possible Association of Glutathione S-Transferase Pi 1 (GSTP1) Gene Polymorphism in Iraqi Patients with Breast Cancer.

IF 1.2 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Reports of Biochemistry and Molecular Biology Pub Date : 2025-01-01 DOI:10.61186/rbmb.13.4.516

Hasan Alhayali, Zohreh Hojati

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引用次数: 0

Abstract

Background: Investigating the role of glutathione S-transferase pi 1 (GSTP1) in breast cancer development and exploring genetic variations in GSTP1 that may contribute to susceptibility to the disease.

Methods: Blood samples were collected from 40 healthy control individuals and 75 breast cancer patients for genomic DNA extraction. PCR and bioinformatics analysis were used to examine the GSTP1 gene sequences.

Results: Gel electrophoresis confirmed the presence of a 433-bp amplified genetic locus with a 212C>A variation in the intron-4 region, identified as rs757152293. The Single nucleotide polymorphisms (SNP) exhibited variable distributions, with homozygous CC and heterozygous CA genotypes. Patient samples with the CA genotype were submitted to NCBI under accession numbers OL957029-OL957036, while those with the CC genotype were submitted under accession numbers OL957027-OL957034. Additionally, sequence analysis of the rs757152293 SNP in healthy individuals was submitted to NCBI under accession numbers OL957037-OL957041.

Conclusions: This study is the first to suggest a possible association between GSTP1 genetic polymorphism and breast cancer in the investigated population.

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谷胱甘肽s -转移酶Pi 1 （GSTP1）基因多态性在伊拉克乳腺癌患者中的可能关联

背景：研究谷胱甘肽s -转移酶pi 1 （GSTP1）在乳腺癌发展中的作用，并探索GSTP1基因变异可能与乳腺癌易感性有关。方法：采集40例健康对照者和75例乳腺癌患者的血液进行基因组DNA提取。采用PCR和生物信息学分析对GSTP1基因序列进行检测。结果：凝胶电泳证实在内含子-4区存在一个433 bp的扩增遗传位点，变异为212C> a，鉴定为rs757152293。单核苷酸多态性（SNP）呈现不同的分布，有纯合子CC基因型和杂合子CA基因型。CA基因型的患者样本提交给NCBI，登记号为OL957029-OL957036， CC基因型的患者样本提交给NCBI，登记号为OL957027-OL957034。此外，健康个体rs757152293 SNP的序列分析已提交给NCBI，登录号为OL957037-OL957041。结论：本研究首次提出在所调查人群中GSTP1基因多态性与乳腺癌之间可能存在关联。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

2.80

自引率

23.50%

发文量

审稿时长

10 weeks

期刊介绍： The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.