[Study on the analgesic effect of electroacupuncture at different intensities on the primary input level in mice with inflammatory muscle pain based on in vivo calcium imaging].
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引用次数: 0
Abstract
Objectives: To observe the differential analgesic effects of electroacupuncture (EA) at different intensities on calcium activity in dorsal root ganglia (DRG) neurons induced by mechanical, thermal, and cold stimuli in a state of muscular inflammatory pain, so as to preliminarily reveal the peripheral integration characteristics of acupuncture analgesia mechanisms.
Methods: The inflammatory muscle pain model was established by injection of complete Freund's adjuvant (CFA) into the right tibialis anterior muscle. EA (0.5 mA or 1 mA, 2 Hz) was applied to "Zusanli" (ST36) on the right side for 10 min. Before and after the EA, mice were given body surface stimulation of brush, clamp and different temperature, while simultaneously recording DRG neuron calcium activity during stimulation. The effects of different stimulation methods before and after EA on the change in fluorescence intensity (ΔF/F0) and the percentage of activated DRG neurons were compared.
Results: In the clamp hind paw stimulation, both 0.5 mA and 1 mA EA could reduce the fluorescence intensity of neurons, and the effect of 1 mA was better than that of 0.5 mA (P<0.001). Specifically, 0.5 mA EA decreased the percentage of medium and large neurons (P<0.05), while 1 mA EA decreased the percentage of small, medium, and large neurons (P<0.05, P<0.01), and the effect of 1 mA was better than that of 0.5 mA in reducing the percentage of small and medium neurons (P<0.01). In the stimulation of clamping tibialis anterior muscle, both EA intensities could reduce the fluorescence intensity of neurons (P<0.05, P<0.000 1), and the effect of 1 mA was better than that of 0.5 mA (P<0.000 1), and 1 mA EA could reduce the percentage of small and medium neurons (P<0.05). In the brush stimulation, both EA intensities could reduce the fluorescence intensity of neurons (P<0.000 1), and the effect of 1 mA was better than that of 0.5 mA (P<0.000 1), and 0.5 mA EA decreased the percentage of medium and large neurons (P<0.05), while 1 mA EA decreased the percentage of small, medium and large neurons (P<0.05, P<0.01). However, in the temperature stimulation (43, 51, 4 ℃), there were no obvious changes in the fluorescence intensity and the percentage of activated neurons in different intensities of EA.
Conclusions: Both 0.5 mA and 1 mA EA intervention at ST36 can inhibit the reactivity of DRG neurons to mechanical stimulation, with higher intensity EA showing more significant effects.
目的：观察不同强度电针（EA）对肌肉炎性疼痛状态下机械、热、冷刺激下背根神经节（DRG）神经元钙活性的差异镇痛作用，初步揭示针刺镇痛机制的外周整合特征。方法：右胫骨前肌注射完全弗氏佐剂（CFA），建立炎性肌肉疼痛模型。在“足三里”（ST36）右侧施加0.5 mA或1 mA， 2 Hz的EA，持续10分钟。在EA前后分别给予小鼠体表刷、钳和不同温度刺激，同时记录刺激过程中DRG神经元钙活性。比较EA前后不同刺激方式对荧光强度（ΔF/F0）变化及DRG神经元活化百分比的影响。结果：在钳后爪刺激中，0.5 mA和1 mA EA均能降低神经元的荧光强度，且1 mA的效果优于0.5 mA （pppppppppppppppppppppppp36）。结论：0.5 mA和1 mA EA干预均能抑制DRG神经元对机械刺激的反应性，且EA强度越高，作用越显著。
期刊介绍：
Acupuncture Research was founded in 1976. It is an acupuncture academic journal supervised by the State Administration of Traditional Chinese Medicine, co-sponsored by the Institute of Acupuncture of the China Academy of Chinese Medical Sciences and the Chinese Acupuncture Association. This journal is characterized by "basic experimental research as the main focus, taking into account clinical research and reporting". It is the only journal in my country that focuses on reporting the mechanism of action of acupuncture.
The journal has been changed to a monthly journal since 2018, published on the 25th of each month, and printed in full color. The manuscript acceptance rate is about 10%, and provincial and above funded projects account for about 80% of the total published papers, reflecting the latest scientific research results in the acupuncture field and has a high academic level. Main columns: mechanism discussion, clinical research, acupuncture anesthesia, meridians and acupoints, theoretical discussion, ideas and methods, literature research, etc.
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