Detection of human papillomavirus in male genital samples.

IF 2.9 2区 医学 Q2 UROLOGY & NEPHROLOGY
Saúl A Villasmil, Jorgen A Marcano, José A Briceño, Alejandro Rísquez, Militza Quintero, John Cruz, Miriam Y Villasmil, Leonardo Acosta, Luis Duarte, César El Kadi
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引用次数: 0

Abstract

Purpose: This study focuses on the detection of human papillomavirus (HPV) in genital samples from heterosexual men, with or without HPV-like lesions, using molecular biology techniques for diagnosis and genotyping of the virus.

Method: A retrospective observational study was conducted with 294 male patients who visited a laboratory for molecular testing, with a presumptive clinical-epidemiological diagnosis of HPV infection, without prior HPV disease or vaccination. Samples were analyzed using Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) techniques.

Results: Detection of HPV-infected men was 50.8%, with significant variability in incidence depending on the anatomical site of sampling. Fourteen high-risk HPV types were identified, along with the presence of multiple subtypes in several patients.

Conclusion: This study provides valuable insights into HPV incidence in men, the most prevalent genotypes, and their correlation with lesion presence. These findings may aid in developing prevention, vaccination, and control strategies for HPV in men.

男性生殖器样本中人乳头瘤病毒的检测。
目的:本研究的重点是在异性恋男性生殖器样本中检测人乳头瘤病毒(HPV),无论有无HPV样病变,使用分子生物学技术进行病毒的诊断和基因分型。方法:对294例男性患者进行回顾性观察性研究,这些患者到实验室进行分子检测,推定为HPV感染的临床流行病学诊断,先前没有HPV疾病或接种疫苗。采用聚合酶链反应(PCR)和限制性片段长度多态性(RFLP)技术对样品进行分析。结果:男性hpv感染者的检出率为50.8%,随取样解剖部位的不同,检出率有显著差异。确定了14种高危型HPV,同时在一些患者中存在多种亚型。结论:本研究为HPV在男性中的发病率、最普遍的基因型及其与病变存在的相关性提供了有价值的见解。这些发现可能有助于制定男性HPV的预防、疫苗接种和控制策略。
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来源期刊
World Journal of Urology
World Journal of Urology 医学-泌尿学与肾脏学
CiteScore
6.80
自引率
8.80%
发文量
317
审稿时长
4-8 weeks
期刊介绍: The WORLD JOURNAL OF UROLOGY conveys regularly the essential results of urological research and their practical and clinical relevance to a broad audience of urologists in research and clinical practice. In order to guarantee a balanced program, articles are published to reflect the developments in all fields of urology on an internationally advanced level. Each issue treats a main topic in review articles of invited international experts. Free papers are unrelated articles to the main topic.
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