Biomechanical implications of lumbar intervertebral disc fenestration in rabbits: Comparison of ex vivo and in vivo conditions as an experimental model for chrondrodystrophic dogs with type 1 intervertebral disc disease.

IF 1.3 2区农林科学 Q2 VETERINARY SCIENCES

Veterinary Surgery Pub Date : 2025-08-21 DOI:10.1111/vsu.14327

James D Crowley, Rema A Oliver, Tian Wang, Matthew H Pelletier, William R Walsh

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Abstract

Objective: To compare the effect of intervertebral disc (IVD) fenestration on L3/4 range of motion (ROM) under ex vivo and in vivo conditions in a rabbit model.

Study design: Randomized experimental study.

Animals: New Zealand White rabbits (ex vivo: n = 18; in vivo: n = 12).

Methods: L3/4 motion segments were tested in lateral bending (LB), flexion-extension (FE), and axial rotation (AR) across five groups (n = 6 per group): control (C), ex vivo fenestration (EVF), in vivo fenestration with a 6 (IVF6) and 12 week (IVF12) timepoint, and intact/annulotomy/fenestration on the same specimen (IAF). Data are reported as ROM and neutral zone (NZ) and were compared between groups using ANOVA and t-tests.

Results: In IAF, fenestration increased ROM in LB, FE, and AR (p = .030) and NZ in FE (p = .010) compared to intact. EVF had greater ROM and NZ than C in all planes (LB, FE: p = .0010; AR: p = .030). IVF6 and IVF12 showed reduced ROM and NZ in LB and FE compared to C (p = .020-.0010) and EVF (p = .0010), with no difference in AR (p = .35). IVF6 and IVF12 did not differ significantly in any plane. Right lateral bending was significantly greater than left in EVF (p = .020), but not in IVF6 (p = .15) or IVF12 (p = .31).

Conclusion: Fenestration increased spinal motion ex vivo, while in vivo specimens showed reduced ROM over time, consistent with a stabilizing biological response.

Clinical significance: These findings provide a foundation for investigating the biological and biomechanical effects of IVD fenestration in dogs.

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兔腰椎间盘开窗的生物力学意义：作为伴有1型椎间盘疾病的慢性营养不良犬的实验模型的离体和体内条件的比较

目的：比较体外和体内条件下椎间盘开窗对兔模型L3/4关节活动度（ROM）的影响。研究设计：随机实验研究。动物：新西兰大白兔（离体18只，体内12只）。方法：对L3/4运动节段进行横向弯曲（LB）、屈伸（FE）和轴向旋转（AR）测试，分为五组（每组n = 6）：对照组(C)、离体开窗（EVF）、6周（IVF6）和12周（IVF12）时间点的体内开窗，以及同一标本的完整/环切/开窗（IAF）。数据报告为ROM和中性区（NZ），并使用方差分析和t检验进行组间比较。结果：在IAF中，与完整组相比，开窗增加了LB、FE和AR的ROM （p = 0.030）和FE的NZ （p = 0.010）。EVF各平面的ROM和NZ均大于C （LB, FE: p = 0.0010; AR: p = 0.030）。与C和EVF相比，IVF6和IVF12显示LB和FE中的ROM和NZ减少（p = 0.020 - 0.0010）。0010)， AR无差异（p = 0.35）。IVF6和IVF12在各平面上无显著差异。EVF右侧弯曲明显大于左侧弯曲(p =。020)，但IVF6 （p = .15）或IVF12 （p = .31）没有。结论：开窗增加了离体脊柱运动，而体内标本随着时间的推移显示ROM减少，与稳定的生物反应一致。临床意义：本研究结果为进一步研究犬IVD开窗的生物学和生物力学效应奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Surgery 农林科学-兽医学

CiteScore

3.40

自引率

22.20%

发文量

162

审稿时长

8-16 weeks

期刊介绍： Veterinary Surgery, the official publication of the American College of Veterinary Surgeons and European College of Veterinary Surgeons, is a source of up-to-date coverage of surgical and anesthetic management of animals, addressing significant problems in veterinary surgery with relevant case histories and observations. It contains original, peer-reviewed articles that cover developments in veterinary surgery, and presents the most current review of the field, with timely articles on surgical techniques, diagnostic aims, care of infections, and advances in knowledge of metabolism as it affects the surgical patient. The journal places new developments in perspective, encompassing new concepts and peer commentary to help better understand and evaluate the surgical patient.