Comparative Detection and Genetic Characterization of Feline Panleukopenia Virus in Bangladesh.

IF 1.7 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Medicine and Science Pub Date : 2025-09-01 DOI:10.1002/vms3.70594

Nurejunnati Jeba, Roni Mia, Md Masum Billah Tarafder, Anandha Mozumder, Raduyan Farazi, S M Nazmul Hasan, Mohammad Bayazid Bostami, A K M Anisur Rahman, Abdul Mannan, Sharmin Akter, Sukumar Saha, Tofazzal Islam, Elcio Leal, Antonio Charlys da Costa, Md Golzar Hossain

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Abstract

Background: Feline panleukopenia virus (FPV) is a highly contagious and often fatal disease affecting domestic and wild felines. Accurate diagnosis and understanding of circulating strains are essential for effective control.

Objectives: This study aimed to evaluate the diagnostic accuracy of a rapid immunochromatographic (IC) antigen test compared to PCR for FPV detection in clinically suspected pet cats in Bangladesh. It also aimed to investigate the genetic and evolutionary characteristics of circulating FPV strains.

Methods: Faecal or rectal swab samples from suspected cats were tested using both IC strip tests and PCR. Sensitivity and specificity of the IC test were analysed using PCR as the reference. Partial sequencing of the VP2 gene was performed on four PCR-positive samples for phylogenetic and mutational analysis. Structural modelling of VP2 proteins was conducted to predict conformational changes.

Results: The IC test detected FPV in 84% of cases, whereas PCR confirmed only 60%, indicating a 24% false-positive rate. PCR showed higher diagnostic reliability. FPV prevalence was 92% among unvaccinated cats. Phylogenetic analysis of VP2 sequences revealed close genetic similarity with Chinese and Portuguese strains, suggesting possible cross-border transmission. Mutations such as A756G, A896G, E299G and T236I were consistently observed. Structural modelling indicated minor conformational changes in VP2.

Conclusion and clinical significance: PCR offers superior accuracy over IC testing for FPV diagnosis. Mutational changes may impact antigenicity and diagnostic performance. Improved diagnostic accuracy, molecular surveillance and updated vaccination strategies are essential to control FPV outbreaks in feline populations.

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孟加拉国猫泛白细胞减少症病毒的比较检测和遗传特征。

背景：猫泛白细胞减少病毒（FPV）是一种影响家养和野生猫科动物的高度传染性和经常致命的疾病。准确诊断和了解流行菌株对有效控制至关重要。目的：本研究旨在评估快速免疫层析（IC）抗原检测与PCR检测在孟加拉国临床疑似宠物猫中FPV的诊断准确性。目的是研究流行口蹄疫毒株的遗传和进化特征。方法：对疑似猫的粪便或直肠拭子样本进行IC条试验和PCR检测。以PCR为参照，分析IC检测的敏感性和特异性。对4个pcr阳性样本进行VP2基因的部分测序，进行系统发育和突变分析。对VP2蛋白进行结构建模以预测构象变化。结果：在84%的病例中，IC检测到FPV，而PCR仅检测到60%，假阳性率为24%。PCR诊断可靠性较高。在未接种疫苗的猫中，FPV患病率为92%。系统发育分析显示，VP2序列与中国和葡萄牙菌株具有密切的遗传相似性，提示可能存在跨界传播。A756G、A896G、E299G、T236I等突变一致。构造模拟显示VP2的构象变化较小。结论及临床意义：PCR诊断FPV的准确性优于IC检测。突变变化可能影响抗原性和诊断性能。提高诊断准确性、分子监测和更新疫苗接种策略对于控制猫科动物种群中的口蹄疫疫情至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Medicine and Science Veterinary-General Veterinary

CiteScore

3.00

自引率

0.00%

发文量

296

期刊介绍： Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science. Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper. We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell. Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.