Early proteomic response in thyroid gland after 131I administration in female Balb/c mice.

IF 0.7 4区环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES

Radiation protection dosimetry Pub Date : 2025-08-26 DOI:10.1093/rpd/ncaf057

Nils Rudqvist, Johan Spetz, Emil Schüler, Toshima Z Parris, Britta Langen, Carina Sihlbom Wallem, Khalil Helou, Eva Forssell-Aronsson

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Abstract

131I (iodide) accumulates in the thyroid and may affect thyroid tissue. Mechanisms behind such effects are not known. The aim was to investigate early changes in protein expression in thyroid and plasma from mice injected with 131I as iodide. Female Balb/c nude mice were i.v. injected with 0 or 490 kBq 131I and killed after 24 h. Thyroid and blood samples were collected from each animal. Protein levels were determined by mass spectrometry. Data are available via ProteomeXchange with identifier PXD062861. Altogether, 17 and 20 proteins showed statistically significant altered levels in thyroid gland and plasma, respectively, after 131I exposure. Most of these proteins had decreased and increased levels in thyroid and plasma, respectively. Few of them were previously proposed radiation responsive proteins. Functional annotation suggests impact on haematopoiesis, reduced oxygen levels, and hypothyroidism. The role of CHIA and PGAM2 in radiation-induced response should be further examined, together with identification and validation of biomarkers of 131I exposure.

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雌性Balb/c小鼠注射131I后甲状腺早期蛋白质组学反应。

碘在甲状腺中蓄积，可能影响甲状腺组织。这种效应背后的机制尚不清楚。目的是研究注射131I作为碘化物的小鼠甲状腺和血浆中蛋白质表达的早期变化。分别给Balb/c雌性裸鼠静脉注射0或490 kBq 131I， 24 h后处死，取甲状腺和血液标本。用质谱法测定蛋白质水平。数据可通过ProteomeXchange获得，标识符为PXD062861。总共有17种和20种蛋白质在131I暴露后分别在甲状腺和血浆中显示有统计学意义的改变。这些蛋白在甲状腺和血浆中的含量分别下降和增加。其中很少有先前提出的辐射反应蛋白。功能注释提示对造血、氧水平降低和甲状腺功能减退的影响。应进一步研究CHIA和PGAM2在辐射诱导反应中的作用，以及131I暴露的生物标志物的鉴定和验证。

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来源期刊

Radiation protection dosimetry 环境科学-公共卫生、环境卫生与职业卫生

CiteScore

1.40

自引率

10.00%

发文量

223

审稿时长

6-12 weeks

期刊介绍： Radiation Protection Dosimetry covers all aspects of personal and environmental dosimetry and monitoring, for both ionising and non-ionising radiations. This includes biological aspects, physical concepts, biophysical dosimetry, external and internal personal dosimetry and monitoring, environmental and workplace monitoring, accident dosimetry, and dosimetry related to the protection of patients. Particular emphasis is placed on papers covering the fundamentals of dosimetry; units, radiation quantities and conversion factors. Papers covering archaeological dating are included only if the fundamental measurement method or technique, such as thermoluminescence, has direct application to personal dosimetry measurements. Papers covering the dosimetric aspects of radon or other naturally occurring radioactive materials and low level radiation are included. Animal experiments and ecological sample measurements are not included unless there is a significant relevant content reason.