Transmission electron microscopy reveals the impact of Or gene on chromoplast morphology and β-carotene accumulation in Or gene introgressed tropical cauliflower.

IF 2.5 3区生物学 Q3 CELL BIOLOGY

Protoplasma Pub Date : 2025-08-29 DOI:10.1007/s00709-025-02106-5

Anamika Chandel, Shrawan Singh

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引用次数: 0

Abstract

Cauliflower Or gene governs massive accumulation of β-carotene in the edible 'curd' portion. However, homozygous dominant (OrOr) plants accumulate higher β-carotene than heterozygous (Oror) individuals, yet this phenomenon has not been thoroughly elucidated in relation to chromoplast morphology. A study was performed in a complete randomised block design with three replicates, in which curd samples of homozygous orange (OrOr; CF_Or-HM)_, heterozygous orange (Oror; CF_Or-HT) and white (oror; CF_WT) genotypes were analysed using transmission electron microscopy (TEM). The number of chromoplasts in a cell and their morphology (shape and size) exhibited significant variation in the genotypes. In CF_Or-HM genotypes, chromoplasts exhibited a membrane-like structure, but in CF_Or-HT, they were small granules. The number of chromoplasts was significantly higher in CF_Or-HM compared to CF_Or-HT. The CF_WT had leucoplasts instead of chromoplasts. The CF_Or-HM (15.1 ± 0.1 µg/g FW) had significantly higher β-carotene content than CF_Or-HT (5.6 ± 0.3 µg/g FW). Both CF_Or-HM and CF_Or-HT had 18 and 7 times higher β-carotene than the white counterpart CF_WT (0.8 ± 0.1 µg/g FW). The number and size of chromoplasts exhibit a strong correlation with the concentration of total carotenoids and β-carotene in the curd portion. This is the first systematic report on changes in chromoplast features associated with Or-gene zygosity in cauliflower.

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透射电镜观察Or基因对Or基因渗入的热带花椰菜叶绿体形态和β-胡萝卜素积累的影响。

或基因控制大量积累的β-胡萝卜素在可食用的“凝乳”部分。然而，纯合子显性（OrOr）植株比杂合子（OrOr）个体积累了更高的β-胡萝卜素，但这种现象与染色质形态的关系尚未完全阐明。本研究采用完全随机区组设计，共3个重复，利用透射电子显微镜（TEM）分析纯合子橙（OrOr; CFOr-HM）、杂合子橙（OrOr; CFOr-HT）和白色（OrOr； CFWT）基因型凝乳样品。细胞中染色质的数量及其形态（形状和大小）在基因型中表现出显著的差异。在CFOr-HM基因型中，染色质呈膜状结构，而在CFOr-HT基因型中，染色质呈小颗粒状。与CFOr-HT相比，CFOr-HM组的染色质数量显著增加。CFWT有白质而没有色质体。CFOr-HM（15.1±0.1µg/g FW）的β-胡萝卜素含量显著高于CFOr-HT（5.6±0.3µg/g FW）。CFOr-HM和CFOr-HT的β-胡萝卜素含量分别是白色CFWT的18倍和7倍（0.8±0.1µg/g FW）。凝乳部分中总类胡萝卜素和β-胡萝卜素的浓度与叶绿体的数量和大小密切相关。本文首次系统地报道了花椰菜中与or基因合子性相关的染色质特征变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Protoplasma 生物-细胞生物学

CiteScore

6.60

自引率

6.90%

发文量

审稿时长

4-8 weeks

期刊介绍： Protoplasma publishes original papers, short communications and review articles which are of interest to cell biology in all its scientific and applied aspects. We seek contributions dealing with plants and animals but also prokaryotes, protists and fungi, from the following fields: cell biology of both single and multicellular organisms molecular cytology the cell cycle membrane biology including biogenesis, dynamics, energetics and electrophysiology inter- and intracellular transport the cytoskeleton organelles experimental and quantitative ultrastructure cyto- and histochemistry Further, conceptual contributions such as new models or discoveries at the cutting edge of cell biology research will be published under the headings "New Ideas in Cell Biology".