Establishment and evaluation of an animal model of acquired tracheomalacia following tracheostomy in rabbits.

IF 1.6 3区医学 Q2 PEDIATRICS

Pediatric Surgery International Pub Date : 2025-08-27 DOI:10.1007/s00383-025-06167-8

Juma Obayashi, Maho Wada, Junki Koike, Kanji Otsubo, Koji Kojima, Shigeyuki Furuta

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引用次数: 0

Abstract

Purpose: Acquired tracheomalacia (TM) following tracheostomy can hinder decannulation and affect the quality of life of pediatric patients. Therefore, a reproducible animal model of type III TM is required for further research and therapeutic development.

Methods: We established a rabbit model of acquired TM by resecting the anterior walls of the 2nd to 4th tracheal cartilage rings, while preserving the mucosa. Bronchoscopic evaluations were conducted at three time points: before surgery (term 1), immediately after surgery (term 2), and 3-4 months post-surgery (term 3). The area of the tracheal lumen was measured under varying negative suction pressures using image analysis.

Results: Five of the six rabbits survived and successfully modeled TM. Progressive luminal narrowing was observed, particularly at term 3, where the area decreased to 15.0 ± 18.8% under 30 cmHg suction. Compared with term 1, the same suction pressures resulted in a greater collapse at terms 2 and 3, indicating gradual structural weakening. No respiratory symptoms were observed during the follow-up.

Conclusion: This rabbit model of acquired type III TM is reproducible and enables the quantitative evaluation of airway collapsibility. This may be useful for investigating TM pathophysiology and testing new treatment strategies.

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兔气管切开术后获得性气管软化动物模型的建立与评价。

目的：小儿气管切开术后获得性气管软化症（TM）会阻碍脱管，影响患者的生活质量。因此，需要一种可重复的III型TM动物模型来进行进一步的研究和治疗开发。方法：切除第2 ~ 4气管软骨环前壁，保留粘膜，建立兔获得性TM模型。在术前（第1期）、术后即刻（第2期）、术后3-4个月（第3期）三个时间点进行支气管镜评估。利用图像分析测量不同负压下气管管腔的面积。结果：6只家兔中5只成活，成功建立TM模型。观察到进行性管腔狭窄，特别是在第3期，在30 cmHg吸力下，面积减少到15.0±18.8%。与第1阶段相比，相同的吸力压力导致第2和第3阶段的崩塌更大，表明结构逐渐减弱。随访期间未见呼吸道症状。结论：获得性III型TM兔模型可重复性好，可定量评价气道溃散性。这可能有助于研究TM的病理生理学和测试新的治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pediatric Surgery International 医学-外科

CiteScore

3.00

自引率

5.60%

发文量

215

审稿时长

3-6 weeks

期刊介绍： Pediatric Surgery International is a journal devoted to the publication of new and important information from the entire spectrum of pediatric surgery. The major purpose of the journal is to promote postgraduate training and further education in the surgery of infants and children. The contents will include articles in clinical and experimental surgery, as well as related fields. One section of each issue is devoted to a special topic, with invited contributions from recognized authorities. Other sections will include: -Review articles- Original articles- Technical innovations- Letters to the editor