Comparative glycomic analysis of hawk (Rupornis magnirostris), caiman (Caiman latirostris) and sea turtle (Caretta caretta) tear films.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular omics Pub Date : 2025-09-02 DOI:10.1039/d4mo00255e

Ana Cláudia Raposo, Sheryl Joyce B Grijaldo-Alvarez, Gege Xu, Michael Russelle S Alvarez, Carlito B Lebrilla, Ricardo Wagner Portela, Arianne Oriá

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引用次数: 0

Abstract

Glycans are recognized as biomarkers and therapeutic targets. However, these molecules remain a critical blind spot in understanding post-translational modifications, particularly in vertebrate species inhabiting diverse habitats. The glycans present in tears play a crucial role in eye protection and may be one of the key factors in adapting to direct environmental contact. This study aimed to describe and compare the glycomic profiles of roadside hawk (Rupornis magnirostris), broad-snouted caiman (Caiman latirostris), and loggerhead sea turtle (Caretta caretta) tears, thereby one avian and two reptilian species. Samples were collected from 10 healthy roadside hawks, 70 broad-snouted caimans, and 10 loggerhead sea turtles to determine N- and O-glycan compounds. The compounds were released from tear glycoproteins and enriched by solid-phase extraction (SPE). Then, the glycans were eluted based on size and polarity. SPE fractions were analyzed using high-resolution mass spectrometry. 155 N-glycans (56% sialylated) and 259 O-glycans (37% sialylated) were detected in roadside hawk tears; 127 N-glycans (55% sialylated) and 263 O-glycans (35% sialofucosylated) in broad-snouted caiman tears; and 85 N-glycans (36% fucosylated) and 84 O-glycans (89% fucosylated) in loggerhead sea turtle tears. The marine habitat has a significant impact on the tear's glycans. The high presence of fucosylated glycans can represent a shield mechanism potentially related to its adhesion to glycocalyx, and interaction with the immune system, also serving as an environmental biomarker. Tears are composed of various biologically active substances, and this description can help in further studies on the identification of novel ocular surface biomarkers and in the differentiation of glycan profiles in healthy and non-healthy animals.

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鹰（Rupornis magnirostris）、凯门鳄（caiman latirostris）和海龟（Caretta Caretta）泪膜的比较糖谱分析。

聚糖是公认的生物标志物和治疗靶点。然而，这些分子仍然是理解翻译后修饰的关键盲点，特别是在生活在不同栖息地的脊椎动物物种中。泪液中存在的聚糖在眼睛保护中起着至关重要的作用，可能是适应直接环境接触的关键因素之一。本研究旨在描述和比较路边鹰（Rupornis magnirostris）、宽嘴凯门鳄（caiman latirostris）和红海龟（Caretta Caretta）的糖谱特征，从而确定一种鸟类和两种爬行动物。研究人员从10只健康的路边鹰、70只宽嘴凯门鳄和10只红海龟身上收集样本，以确定N-和o -聚糖化合物。这些化合物从泪液糖蛋白中释放出来，并通过固相萃取（SPE）富集。然后，根据聚糖的大小和极性进行洗脱。采用高分辨率质谱法分析固相萃取馏分。路边鹰泪液中检测到155个n -聚糖（56%唾液化）和259个o -聚糖（37%唾液化）；宽口凯门鳄泪中含有127个n -聚糖（55%唾液化）和263个o -聚糖（35%唾液化）；红海龟眼泪中有85个n -聚糖（36%浓缩）和84个o -聚糖（89%浓缩）。海洋生境对泪液的聚糖有显著影响。高聚焦聚糖的存在可以代表一种潜在的与糖萼粘附和与免疫系统相互作用相关的屏蔽机制，也可以作为一种环境生物标志物。泪液是由多种生物活性物质组成的，这种描述有助于进一步研究新的眼表生物标志物的鉴定以及健康和非健康动物的聚糖谱的分化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular omics Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

5.40

自引率

3.40%

发文量

期刊介绍： Molecular Omics publishes high-quality research from across the -omics sciences. Topics include, but are not limited to: -omics studies to gain mechanistic insight into biological processes – for example, determining the mode of action of a drug or the basis of a particular phenotype, such as drought tolerance -omics studies for clinical applications with validation, such as finding biomarkers for diagnostics or potential new drug targets -omics studies looking at the sub-cellular make-up of cells – for example, the subcellular localisation of certain proteins or post-translational modifications or new imaging techniques -studies presenting new methods and tools to support omics studies, including new spectroscopic/chromatographic techniques, chip-based/array technologies and new classification/data analysis techniques. New methods should be proven and demonstrate an advance in the field. Molecular Omics only accepts articles of high importance and interest that provide significant new insight into important chemical or biological problems. This could be fundamental research that significantly increases understanding or research that demonstrates clear functional benefits. Papers reporting new results that could be routinely predicted, do not show a significant improvement over known research, or are of interest only to the specialist in the area are not suitable for publication in Molecular Omics.