Degradation of Extra-Heavy Crude Oil by Fungi Isolated from Hydrothermal Vents Fields in the Gulf of California.

IF 2 4区 生物学 Q2 AGRONOMY
Mycobiology Pub Date : 2025-08-24 eCollection Date: 2025-01-01 DOI:10.1080/12298093.2025.2545629
Lucia Romero-Hernández, Itandehui Betanzo-Gutiérrez, Rafael Vazquez-Duhalt, Mario Figueroa, Meritxell Riquelme, Patricia Velez
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引用次数: 0

Abstract

Microorganisms from hydrothermal vents fields in the Pescadero Basin represent a promising source for biotechnological applications due to their adaptation to extreme environments. The hydrocarbon seeps in the Pescadero Basin provide a natural precedent for native fungi capable of biodegrading crude oil. In this study, the ability of eight fungal isolates from hydrothermal vent sediments in the Pescadero Basin to bioconvert extra-heavy crude oil (EHCO) was evaluated. Among the isolates, Penicillium sp. 1, Penicillium miczynskii, and Aspergillus terreus degraded different percentages of EHCO, as assessed by gravimetry. Aspergillus sydowii showed the best performance, degrading 40% of EHCO, while Aspergillus sp. 4 showed no significant EHCO degradation. Fungal interaction with EHCO was further explored using scanning electron microscope (SEM). The mycelial growth on EHCO as the sole carbon source was confirmed through the determination of intracellular protein, which was compared to controls lacking a carbon source. Additionally, GC analysis of the saturate fraction of EHCO revealed that A. sydowii degraded 38.26% of it, highlighting its potential as a promising bioremediation agent.

加利福尼亚湾热液喷口区分离真菌降解超重原油的研究。
来自Pescadero盆地热液喷口的微生物因其对极端环境的适应能力而成为生物技术应用的一个有希望的来源。Pescadero盆地的烃类渗漏为能够生物降解原油的本地真菌提供了一个天然的先例。本研究对从Pescadero盆地热液喷口沉积物中分离的8种真菌进行了生物转化超稠油(EHCO)的能力评价。用重量法测定菌株中1号青霉、miczynski青霉和陆地曲霉降解EHCO的比例不同。sydowii曲霉对EHCO的降解效果最好,达到40%,而Aspergillus sp. 4对EHCO的降解效果不显著。利用扫描电镜进一步研究了真菌与EHCO的相互作用。通过测定细胞内蛋白,证实了EHCO作为唯一碳源的菌丝生长,并将其与缺乏碳源的对照进行了比较。此外,对EHCO的饱和组分进行气相色谱分析,结果表明,a . sydowii对EHCO的降解率为38.26%,显示了其作为生物修复剂的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Mycobiology
Mycobiology AGRONOMYMYCOLOGY-MYCOLOGY
CiteScore
3.90
自引率
5.30%
发文量
41
审稿时长
22 weeks
期刊介绍: Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.
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