Uncovering a stable QTL qSRI.A06 and candidate gene for rapeseed pod shatter resistance.

IF 3 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2025-08-22 eCollection Date: 2025-09-01 DOI:10.1007/s11032-025-01590-0

Wenxiang Wang, Wen Chu, Hui Wang, Mei Han, Wei Wang, Hongtao Cheng, Desheng Mei, Qiong Hu, Taocui Huang, Jia Liu, Taocui Huang

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引用次数: 0

Abstract

Rapeseed pods are prone to dehiscence, resulting in yield loss at maturity. In the present study, we investigated the shatter resistance index (SRI) of 280 doubled haploid (DH) lines derived from a cross between ZS11 (susceptible line) and R11 (resistant line). Based on the phenotypic data obtained from four environments and a high-density genetic map, a significant QTL (qSRI.A06) for shatter resistance on A06 chromosome were stably detected. This locus explained 4.80% - 15.00% of the phenotypic variation and the peak position covered a 664 Kb region. The effect of qSRI.A06 was verified in BC₃F₂ and BC₃F₃ populations and delimited in a 90.8 Kb region comprising 11 genes. Out of these genes, a differencially expressed gene, BnaA06g27900D, was identified to be involved in cell wall development by comparative transcriptome analysis. Regional association revealed four SNP/Indel variations in the promoter associated with pod shattering resistance. The highest Indels A06-41975887 showed suggestive association with SRI (p = 8.80E-06) with a TG allele variation. The stable locus qSRI.A06 and the candidate gene BnaA06g27900D will be helpful for understanding the resistance mechanism and improving shatter resistance in rapeseed.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01590-0.

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发现一个稳定的QTL qSRI。油菜籽抗碎荚性候选基因A06。

油菜籽荚容易开裂，在成熟时造成产量损失。研究了由ZS11（易感系）和R11（抗性系）杂交而成的280个双单倍体（DH）抗粉碎指数（SRI）。根据4种环境的表型数据和高密度遗传图谱，在A06染色体上稳定检测到一个抗破碎性显著QTL （qSRI.A06）。该位点解释了4.80% ~ 15.00%的表型变异，峰位覆盖664 Kb区域。qSRI的效果。A06在BC3F2和BC3F3群体中得到验证，划分在90.8 Kb的区域，包含11个基因。在这些基因中，通过比较转录组分析确定了一个差异表达基因BnaA06g27900D参与细胞壁发育。区域关联揭示了与荚果破碎抗性相关的启动子的4个SNP/Indel变异。最高的指数A06-41975887与TG等位基因变异有相关性（p = 8.80E-06）。稳定位点qSRI。A06和候选基因BnaA06g27900D将有助于了解油菜抗碎性机理和提高油菜抗碎性。补充资料：在线版本提供补充资料，网址为10.1007/s11032-025-01590-0。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.