Fascin-1 expression is associated with EMT and recurrence in thymic carcinoma: a clinicopathologic study.

IF 1.1 4区医学 Q3 PATHOLOGY

Medical Molecular Morphology Pub Date : 2025-09-01 DOI:10.1007/s00795-025-00446-y

Hironobu Okada, Yoshihiro Hayashi, Naoki Furukawa, Yujirou Bunno, Marino Yamamoto, Ryohei Miyazaki, Mitsuko Iguchi, Makoto Toi, Ichiro Murakami, Masaya Tamura

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引用次数: 0

Abstract

Fascin-1, an actin-bundling protein, plays a crucial role in cancer cell motility, invasion, and epithelial-mesenchymal transition (EMT). While its significance has been demonstrated in several malignancies, its clinical relevance in thymic carcinoma remains unclear. We retrospectively analyzed 10 surgically resected thymic carcinoma cases treated at Kochi University Hospital from 2008 to 2024. Immunohistochemical staining was performed to evaluate Fascin-1 expression using the Allred scoring system. Expression of EMT-related markers (TGF-β, pSmad3, Snail, and E-cadherin) was also assessed. Associations between Fascin-1 expression and postoperative recurrence were analyzed. Fascin-1 was highly expressed in tumor regions with low E-cadherin expression and co-localized with EMT-inducing markers such as TGF-β and Snail. Patients with postoperative recurrence showed significantly higher Fascin-1 scores than those without recurrence (P = 0.048). Double immunofluorescence confirmed an inverse relationship between Fascin-1 and E-cadherin expression. High Fascin-1 expression may serve as a potential marker for postoperative recurrence in thymic carcinoma and is closely associated with EMT. These findings suggest Fascin-1 as a promising prognostic biomarker and therapeutic target, although further studies with larger cohorts and functional analyses are warranted.

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胸腺癌中筋膜蛋白-1表达与EMT和复发相关：一项临床病理研究

fasin -1是一种肌动蛋白结合蛋白，在癌细胞的运动、侵袭和上皮间质转化（EMT）中起着至关重要的作用。虽然其在几种恶性肿瘤中的重要性已得到证实，但其在胸腺癌中的临床意义尚不清楚。我们回顾性分析2008年至2024年在高知大学医院手术切除的10例胸腺癌病例。采用Allred评分系统进行免疫组化染色，评估fastin -1的表达。emt相关标志物（TGF-β、pSmad3、Snail和E-cadherin）的表达也被评估。分析了fasin -1表达与术后复发的关系。fasin -1在E-cadherin低表达的肿瘤区域高表达，并与TGF-β、Snail等emt诱导标志物共定位。术后复发患者的fasin -1评分明显高于无复发患者（P = 0.048）。双免疫荧光证实了fasin -1与E-cadherin表达呈负相关。高fasin -1表达可能是胸腺癌术后复发的潜在标志，与EMT密切相关。这些发现表明，尽管有必要进行更大规模的研究和功能分析，但筋膜蛋白-1作为一种有希望的预后生物标志物和治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Medical Molecular Morphology 医学-病理学

CiteScore

2.90

自引率

5.60%

发文量

审稿时长

>12 weeks

期刊介绍： Medical Molecular Morphology is an international forum for researchers in both basic and clinical medicine to present and discuss new research on the structural mechanisms and the processes of health and disease at the molecular level. The structures of molecules, organelles, cells, tissues, and organs determine their normal function. Disease is thus best understood in terms of structural changes in these different levels of biological organization, especially in molecules and molecular interactions as well as the cellular localization of chemical components. Medical Molecular Morphology welcomes articles on basic or clinical research in the fields of cell biology, molecular biology, and medical, veterinary, and dental sciences using techniques for structural research such as electron microscopy, confocal laser scanning microscopy, enzyme histochemistry, immunohistochemistry, radioautography, X-ray microanalysis, and in situ hybridization. Manuscripts submitted for publication must contain a statement to the effect that all human studies have been reviewed by the appropriate ethics committee and have therefore been performed in accordance with the ethical standards laid down in an appropriate version of the 1964 Declaration of Helsinki. It should also be stated clearly in the text that all persons gave their informed consent prior to their inclusion in the study. Details that might disclose the identity of the subjects under study should be omitted.