Ning Wang, Han Yang, Xue Tong, Tong Xu, Jiamin Zhao, Yi-Kai Li
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引用次数: 0
Abstract
Scope: Estrogen deficiency post-menopause is a key driver of bone loss and is often associated with disruption of the gut-bone axis. This study explored the therapeutic potential of Ginkgolide B (GB), a natural bioactive compound from Ginkgo biloba, in estrogen deficiency-induced bone loss using ovariectomized (OVX) mice..
Methods: Female SPF C57BL/6J mice were randomly divided into sham-operated (Sham), OVX, and OVX+GB groups. Osteoporosis was induced by bilateral ovariectomy, and GB was administered via oral gavage for 8 weeks. Evaluations included serum biochemical markers, bone microstructure (micro-CT, histomorphometry), intestinal barrier function (histology, immunohistochemistry, and Western blot), immune cell populations (flow cytometry), and gut microbiota composition (16S rRNA gene sequencing).
Results: GB treatment significantly mitigated bone loss in OVX mice, as confirmed by micro-CT, histological, and biochemical tests. GB enhanced intestinal barrier integrity by upregulating tight junction proteins and reducing pro-inflammatory markers. Flow cytometry showed that GB restored the balance of T helper 17 (Th17) and regulatory T (Treg) cells in the mesenteric lymph node and spleen, suggesting immune regulation. Additionally, GB modulated gut microbiota composition, increasing beneficial taxa like Lactobacillaceae and Prevotellaceae. Correlation analysis indicated significant associations between microbial taxa and serum markers and bone metabolism, highlighting the role of gut microbiota in GB's therapeutic effects on osteoporosis.
Conclusion: GB is a promising natural bioactive compound for modulating bone health through the gut-bone axis, warranting further research to clarify underlying mechanisms.
期刊介绍：
Journal of Orthopaedic Surgery and Research is an open access journal that encompasses all aspects of clinical and basic research studies related to musculoskeletal issues.
Orthopaedic research is conducted at clinical and basic science levels. With the advancement of new technologies and the increasing expectation and demand from doctors and patients, we are witnessing an enormous growth in clinical orthopaedic research, particularly in the fields of traumatology, spinal surgery, joint replacement, sports medicine, musculoskeletal tumour management, hand microsurgery, foot and ankle surgery, paediatric orthopaedic, and orthopaedic rehabilitation. The involvement of basic science ranges from molecular, cellular, structural and functional perspectives to tissue engineering, gait analysis, automation and robotic surgery. Implant and biomaterial designs are new disciplines that complement clinical applications.
JOSR encourages the publication of multidisciplinary research with collaboration amongst clinicians and scientists from different disciplines, which will be the trend in the coming decades.
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