Potential of Tomato Seed Extract for Wrinkle Care through Human Neutrophil Elastase Inhibition: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial.

IF 1.1 4区 医学 Q4 NUTRITION & DIETETICS
Tomomi Degawa, Shogo Takeda, Norihito Shimizu, Kenchi Miyasaka, Hiroshi Shimoda, Hirotaka Miyazaki, Akihiro Tada
{"title":"Potential of Tomato Seed Extract for Wrinkle Care through Human Neutrophil Elastase Inhibition: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial.","authors":"Tomomi Degawa, Shogo Takeda, Norihito Shimizu, Kenchi Miyasaka, Hiroshi Shimoda, Hirotaka Miyazaki, Akihiro Tada","doi":"10.3177/jnsv.71.366","DOIUrl":null,"url":null,"abstract":"<p><p>Numerous cosmetic products claim to improve aging-induced wrinkles, and recent research has explored the potential of oral intake for wrinkle improvement. In our previous study, we investigated the effects of tomato seed extract (TSE), which contains lycoperoside H, on skin elasticity and its anti-inflammatory properties. The present study aimed to explore the anti-wrinkle effects of TSE through the inhibition of human neutrophil elastase (HNE), activated by inflammatory stimuli. The results indicated that TSE inhibits HNE (half maximal inhibitory concentration [IC<sub>50</sub>]: 316 μg/mL), and that lycoperoside H contributes to this activity (IC<sub>50</sub>: 37 mM). Additionally, several flavonoids found in TSE have been reported to exhibit HNE inhibitory effects. Based on these findings, we hypothesized that TSE supplementation could suppress excessive HNE activity in wrinkle-prone areas and potentially improve facial wrinkles. Therefore, we conducted a randomized, double-blind, placebo-controlled clinical trial with 45 healthy Japanese women (age range, 40-59 y) who had wrinkle grades of 3-5 around their eyes. The participants were randomly assigned to either a 200 mg/d TSE supplement (containing 1 mg of lycoperoside H) or a placebo for 12 wk. As a result, significant improvement in wrinkle grade was observed in the TSE group; however, improvements were also seen in the placebo group. Therefore, no significant differences in wrinkle reduction were found between the two groups. These findings suggest that the inhibition of HNE by TSE may have potential effects on the dermis, thereby offering new insights into the role of TSE in skin health.</p>","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"71 4","pages":"366-374"},"PeriodicalIF":1.1000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of nutritional science and vitaminology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3177/jnsv.71.366","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
引用次数: 0

Abstract

Numerous cosmetic products claim to improve aging-induced wrinkles, and recent research has explored the potential of oral intake for wrinkle improvement. In our previous study, we investigated the effects of tomato seed extract (TSE), which contains lycoperoside H, on skin elasticity and its anti-inflammatory properties. The present study aimed to explore the anti-wrinkle effects of TSE through the inhibition of human neutrophil elastase (HNE), activated by inflammatory stimuli. The results indicated that TSE inhibits HNE (half maximal inhibitory concentration [IC50]: 316 μg/mL), and that lycoperoside H contributes to this activity (IC50: 37 mM). Additionally, several flavonoids found in TSE have been reported to exhibit HNE inhibitory effects. Based on these findings, we hypothesized that TSE supplementation could suppress excessive HNE activity in wrinkle-prone areas and potentially improve facial wrinkles. Therefore, we conducted a randomized, double-blind, placebo-controlled clinical trial with 45 healthy Japanese women (age range, 40-59 y) who had wrinkle grades of 3-5 around their eyes. The participants were randomly assigned to either a 200 mg/d TSE supplement (containing 1 mg of lycoperoside H) or a placebo for 12 wk. As a result, significant improvement in wrinkle grade was observed in the TSE group; however, improvements were also seen in the placebo group. Therefore, no significant differences in wrinkle reduction were found between the two groups. These findings suggest that the inhibition of HNE by TSE may have potential effects on the dermis, thereby offering new insights into the role of TSE in skin health.

番茄籽提取物通过抑制人体中性粒细胞弹性酶治疗皱纹的潜力:一项随机、双盲、安慰剂对照的临床试验。
许多化妆品声称可以改善衰老引起的皱纹,最近的研究已经探索了口服改善皱纹的潜力。在我们之前的研究中,我们研究了含有番茄苷H的番茄籽提取物(TSE)对皮肤弹性和抗炎特性的影响。本研究旨在探讨TSE通过抑制炎症刺激激活的人中性粒细胞弹性酶(HNE)的抗皱作用。结果表明,TSE对HNE有抑制作用(半数最大抑制浓度[IC50]: 316 μg/mL),番茄苷H对其有抑制作用(IC50: 37 mM)。此外,据报道,在TSE中发现的几种类黄酮具有HNE抑制作用。基于这些发现,我们假设补充TSE可以抑制皱纹易发区域过度的HNE活动,并可能改善面部皱纹。因此,我们对45名健康的日本女性(年龄在40-59岁之间)进行了一项随机、双盲、安慰剂对照的临床试验,这些女性的眼周皱纹等级为3-5。参与者被随机分配到200毫克/天的TSE补充剂(含有1毫克番茄苷H)或安慰剂12周。结果,TSE组皱纹等级显著改善;然而,安慰剂组的情况也有所改善。因此,在皱纹减少两组之间没有发现显著差异。这些发现表明,TSE对HNE的抑制可能对真皮层有潜在的影响,从而为TSE在皮肤健康中的作用提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
1.80
自引率
6.20%
发文量
63
审稿时长
6-12 weeks
期刊介绍: The Journal of Nutritional Science and Vitaminology is an international medium publishing in English of original work in all branches of nutritional science, food science and vitaminology from any country. Manuscripts submitted for publication should be as concise as possible and must be based on the results of original research or of original interpretation of existing knowledge not previously published. Although data may have been reported, in part, in preliminary or abstract form, a full report of such research is unacceptable if it has been or will be submitted for consideration by another journal.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信