Investigation of the Inhibitory Effects of Fatty Acid Derivatives on URAT1 Function, a Renal Urate Re-Absorber.

IF 1.1 4区 医学 Q4 NUTRITION & DIETETICS
Yu Toyoda, Hiroki Saito, Hiroshi Hirata, Ami Ota-Kontani, Youichi Tsuchiya, Tappei Takada
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Abstract

The global increase in hyperuricemia, a pathological condition characterized by elevated serum urate concentrations, emphasizes the importance of appropriate management of uric acid homeostasis in the body. Enhancing renal urate excretion is clinically relevant to achieve serum urate-lowering, and the functional inhibition of urate transporter 1 (URAT1), a renal urate transporter involved in the reabsorption of urate, has been recognized as a promising strategy. In this context, natural substances, including food ingredients with URAT1-inhibitory activity, have garnered significant interest. A previous study demonstrated that various fatty acids, including α-linolenic acid (α-LA), inhibit URAT1; however, further investigations are required to expand our understanding for this important topic. The present study focused on certain metabolites derived from α-LA, especially jasmonates (lipid-derived cyclopentanone compounds in plants) and related substances, and investigated their effects on URAT1-mediated urate transport activity, using a mammalian cell-based functional assay system. Among the tested substances (14 authentic chemicals), 12-oxo-phytodienoic acid (a precursor of jasmonic acid harboring a cyclopentenone ring with two carbon chains in its structure) showed a good URAT1-inhibitory activity with the half maximal inhibitory concentration (IC50) value of 15.9 μM. Comparable results were obtained with prostaglandin A1 (PGA1) and PGA2, which are known as cyclopentenone PGs, that exhibited IC50 values of 22.5 μM and 16.8 μM, respectively. Although further studies are required to address the effects of these substances on the urate regulation in humans, our findings contribute to a deeper understanding of the interactions between URAT1 and natural substances.

脂肪酸衍生物对肾尿酸再吸收剂URAT1功能抑制作用的研究。
高尿酸血症是一种以血清尿酸浓度升高为特征的病理状态,全球高尿酸血症的增加强调了适当管理体内尿酸稳态的重要性。增强肾脏尿酸排泄与降低血清尿酸水平具有临床相关性,而抑制尿酸转运蛋白1 (URAT1)是一种参与尿酸重吸收的肾尿酸转运蛋白,已被认为是一种很有前景的策略。在这种情况下,天然物质,包括具有urat1抑制活性的食品成分,已经引起了极大的兴趣。先前的研究表明,多种脂肪酸,包括α-亚麻酸(α-LA),抑制URAT1;然而,需要进一步的研究来扩大我们对这一重要主题的理解。本研究主要研究α-LA衍生的某些代谢产物,特别是茉莉酸盐(植物中脂质衍生的环戊酮化合物)及其相关物质,并利用基于哺乳动物细胞的功能测定系统研究它们对urat1介导的尿酸盐转运活性的影响。其中,12-氧-植物二烯酸(茉莉酸的前体,含有两个碳链的环戊酮环)具有较好的urat1抑制活性,其半数最大抑制浓度(IC50)为15.9 μM。前列腺素A1 (PGA1)和PGA2(被称为环戊酮pggs)的IC50值分别为22.5 μM和16.8 μM,也获得了类似的结果。虽然需要进一步的研究来解决这些物质对人类尿酸调节的影响,但我们的发现有助于更深入地了解URAT1与天然物质之间的相互作用。
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来源期刊
CiteScore
1.80
自引率
6.20%
发文量
63
审稿时长
6-12 weeks
期刊介绍: The Journal of Nutritional Science and Vitaminology is an international medium publishing in English of original work in all branches of nutritional science, food science and vitaminology from any country. Manuscripts submitted for publication should be as concise as possible and must be based on the results of original research or of original interpretation of existing knowledge not previously published. Although data may have been reported, in part, in preliminary or abstract form, a full report of such research is unacceptable if it has been or will be submitted for consideration by another journal.
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