Bacillus subtilis pb2441 Ameliorates Hepatic Steatosis by Decoupling Liver and Fat Tissue Lipid Accumulation in a High-Fat Diet-Fed Mouse Model.

IF 2 3区 农林科学 Q4 CHEMISTRY, MEDICINAL
Jung-Yun Lee, Sung-Su Park, Il Kyu Cho, Kyoung-Sik Moon, Yangrae Cho
{"title":"<i>Bacillus subtilis</i> pb2441 Ameliorates Hepatic Steatosis by Decoupling Liver and Fat Tissue Lipid Accumulation in a High-Fat Diet-Fed Mouse Model.","authors":"Jung-Yun Lee, Sung-Su Park, Il Kyu Cho, Kyoung-Sik Moon, Yangrae Cho","doi":"10.1177/1096620X251372435","DOIUrl":null,"url":null,"abstract":"<p><p>Nonalcoholic fatty liver disease (NAFLD) is a global health issue, often associated with gut dysbiosis. In recent years, probiotics have gained attention as potential therapeutic agents for NAFLD. This study explored the effects of a single strain, <i>Bacillus subtilis</i> with high surfactin secretion, on C57BL/6 mice fed a high-fat diet (HFD), a model for NAFLD, for 13 weeks. We conducted efficacy assays over 13 weeks on liver fat accumulation and gut microbiome modulation. <i>Bacillus</i> supplementation reduced body weight gain and fat accumulation in the liver, but not in adipose tissues. This indicates a decoupling of hepatic and adipose lipid accumulation-meaning that lipid reduction occurred selectively in the liver, independent of changes in peripheral fat storage. Hepatic steatosis and liver enzyme levels were significantly improved. The supplementation largely maintained or amplified the bacterial abundance shifts caused by the HFD. Only seven-including <i>Lactobacillus</i>, <i>Akkermansia</i>, and <i>Romboutsia</i>-out of 53 bacterial genera which were significantly changed by HFD were restored to normal levels by the supplementation. These three genera are commonly regarded as beneficial for human health due to their roles in gut barrier integrity, immune modulation, and metabolic regulation. In contrast, despite these limited changes in bacterial composition, bacterial enzyme analysis suggested significant metabolic modulation by <i>Bacillus</i> supplementation. A single strain of <i>Bacillus subtilis</i>, instead of a mixture of multiple bacterial strains, can prevent hepatic steatosis without affecting fat tissue weight, underscoring its potential as a targeted therapeutic option through microbiome modulation of a few beneficial bacteria. [Figure: see text].</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of medicinal food","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1177/1096620X251372435","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 0

Abstract

Nonalcoholic fatty liver disease (NAFLD) is a global health issue, often associated with gut dysbiosis. In recent years, probiotics have gained attention as potential therapeutic agents for NAFLD. This study explored the effects of a single strain, Bacillus subtilis with high surfactin secretion, on C57BL/6 mice fed a high-fat diet (HFD), a model for NAFLD, for 13 weeks. We conducted efficacy assays over 13 weeks on liver fat accumulation and gut microbiome modulation. Bacillus supplementation reduced body weight gain and fat accumulation in the liver, but not in adipose tissues. This indicates a decoupling of hepatic and adipose lipid accumulation-meaning that lipid reduction occurred selectively in the liver, independent of changes in peripheral fat storage. Hepatic steatosis and liver enzyme levels were significantly improved. The supplementation largely maintained or amplified the bacterial abundance shifts caused by the HFD. Only seven-including Lactobacillus, Akkermansia, and Romboutsia-out of 53 bacterial genera which were significantly changed by HFD were restored to normal levels by the supplementation. These three genera are commonly regarded as beneficial for human health due to their roles in gut barrier integrity, immune modulation, and metabolic regulation. In contrast, despite these limited changes in bacterial composition, bacterial enzyme analysis suggested significant metabolic modulation by Bacillus supplementation. A single strain of Bacillus subtilis, instead of a mixture of multiple bacterial strains, can prevent hepatic steatosis without affecting fat tissue weight, underscoring its potential as a targeted therapeutic option through microbiome modulation of a few beneficial bacteria. [Figure: see text].

枯草芽孢杆菌pb2441通过解耦肝脏和脂肪组织脂质积累改善高脂饮食喂养小鼠模型中的肝脏脂肪变性
非酒精性脂肪性肝病(NAFLD)是一个全球性的健康问题,通常与肠道生态失调有关。近年来,益生菌作为NAFLD的潜在治疗药物受到了人们的关注。本研究探讨了一株高表面素分泌的枯草芽孢杆菌(Bacillus subtilis)对饲喂高脂肪饮食(HFD)的C57BL/6小鼠(NAFLD模型)13周的影响。我们对肝脏脂肪积累和肠道微生物调节进行了为期13周的疗效分析。补充芽孢杆菌减少了体重增加和肝脏脂肪堆积,但没有减少脂肪组织。这表明肝脏和脂肪脂质积累的解耦-意味着脂质减少选择性地发生在肝脏中,独立于外周脂肪储存的变化。肝脂肪变性和肝酶水平显著改善。这种补充在很大程度上维持或放大了由HFD引起的细菌丰度变化。在被HFD显著改变的53个细菌属中,只有7个(包括Lactobacillus, Akkermansia和romboutsia)在补充后恢复到正常水平。由于它们在肠道屏障完整性、免疫调节和代谢调节方面的作用,这三个属通常被认为对人类健康有益。相比之下,尽管细菌组成的变化有限,但细菌酶分析表明,芽孢杆菌的补充显著调节了代谢。单一枯草芽孢杆菌菌株,而不是多种细菌菌株的混合物,可以在不影响脂肪组织重量的情况下预防肝脂肪变性,强调其作为一种通过微生物组调节一些有益细菌的靶向治疗选择的潜力。[图:见正文]。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of medicinal food
Journal of medicinal food 医学-食品科技
CiteScore
4.50
自引率
0.00%
发文量
154
审稿时长
4.5 months
期刊介绍: Journal of Medicinal Food is the only peer-reviewed journal focusing exclusively on the medicinal value and biomedical effects of food materials. International in scope, the Journal advances the knowledge of the development of new food products and dietary supplements targeted at promoting health and the prevention and treatment of disease.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信