Brazilian Green Propolis Regulates Thermogenesis via Two Distinct Pathways.

IF 1.1 4区医学 Q4 NUTRITION & DIETETICS

Journal of nutritional science and vitaminology Pub Date : 2025-01-01 DOI:10.3177/jnsv.71.375

Takuma Hyodo, Nobuaki Okumura, Takanori Tsuda

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Abstract

Brazilian green propolis (BGP), produced by honey bees, induces beige adipocyte formation and accelerates whole-body energy metabolism. Artepillin C (ArtC), the major cinnamic acid derivative of BGP, induces beige adipocyte formation and increases thermogenesis in inguinal white adipose tissue. However, the effects of BGP on beige adipocyte formation and energy metabolism remain unclear. Therefore, we aimed to examine the effects of BGP on beige adipocyte formation and thermogenesis. We used in vitro and in vivo approaches, such as cell culture and differentiation, component testing, thermogenesis measurement, and calcium influx assay. First, ArtC was confirmed to be a major BGP component contributing to beige adipocyte formation in vitro and in vivo. BGP-induced interscapular brown adipose tissue (iBAT) thermogenesis was not observed upon ArtC stimulation. Thereafter, inhibitory studies revealed that iBAT thermogenesis could be related to the transient receptor potential vanilloid 1 (TRPV1)-sympathetic nervous system channel. Finally, we identified culifolin, a cinnamic acid derivative of BGP, as an active component of this response, which activates calcium signaling in TRPV1-expressing cells. In addition, culifolin administration significantly increased the mouse iBAT temperature to the same extent as increased by BGP. Thus, BGP has a unique biological function in activating the two distinct pathways of brown-adipocyte and beige-adipocyte thermogenesis, contributing to increased energy expenditure.

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巴西绿蜂胶通过两种不同的途径调节产热。

巴西绿蜂胶（BGP），由蜜蜂产生，诱导米色脂肪细胞形成，加速全身能量代谢。Artepillin C （ArtC）是BGP的主要肉桂酸衍生物，可诱导米黄色脂肪细胞形成并增加腹股沟白色脂肪组织的生热作用。然而，BGP对米黄色脂肪细胞形成和能量代谢的影响尚不清楚。因此，我们旨在研究BGP对米黄色脂肪细胞形成和产热的影响。我们使用了体外和体内方法，如细胞培养和分化、成分测试、产热测量和钙内流测定。首先，ArtC被证实是体外和体内促进米色脂肪细胞形成的主要BGP成分。在ArtC刺激下未观察到bp诱导的肩胛间棕色脂肪组织（iBAT）生热作用。此后，抑制研究表明iBAT产热可能与瞬时受体电位香草样蛋白1 (TRPV1)-交感神经系统通道有关。最后，我们确定了桂皮素，一种BGP的肉桂酸衍生物，作为这种反应的活性成分，它激活了trpv1表达细胞中的钙信号。此外，culifolin可显著提高小鼠iBAT温度，其升高程度与BGP相同。因此，BGP在激活棕色脂肪细胞和米色脂肪细胞两种不同的产热途径方面具有独特的生物学功能，有助于增加能量消耗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of nutritional science and vitaminology 医学-营养学

CiteScore

1.80

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Nutritional Science and Vitaminology is an international medium publishing in English of original work in all branches of nutritional science, food science and vitaminology from any country. Manuscripts submitted for publication should be as concise as possible and must be based on the results of original research or of original interpretation of existing knowledge not previously published. Although data may have been reported, in part, in preliminary or abstract form, a full report of such research is unacceptable if it has been or will be submitted for consideration by another journal.