Effects of mixed polyphenol supplementation on lower airway inflammation in horses with Equine Asthma Syndrome

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science Pub Date : 2025-08-30 DOI:10.1016/j.jevs.2025.105681

K.M. Andrews, L.J. Berghaus, K.A. Hart

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引用次数: 0

Abstract

Background

Equine Asthma Syndrome (EAS) is a chronic inflammatory respiratory disease of adult horses. While dietary polyphenols have shown anti-inflammatory effects in human asthma, their use in equine asthma has not been studied.

Aims

To evaluate the effects of a mixed polyphenol supplement on systemic and pulmonary inflammation, bronchoalveolar lavage (BAL) cytology, and clinical respiratory signs in healthy and asthmatic horses. We hypothesized that polyphenol supplementation would decrease systemic and pulmonary inflammation in both healthy and asthmatic horses and would improve BAL cytology and clinical respiratory signs in asthmatic horses.

Methods

In Part One, 18 horses (eight healthy, 10 asthmatic) received a daily mixed polyphenol supplement for 6–8 weeks. Physical examination, BAL cytology, and cytokine concentrations in serum and BAL fluid were assessed before and after supplementation. In Part Two, 10 asthmatic horses were randomized into supplemented and control groups (n = 5/group). After 4 weeks on a low-dust diet with or without supplementation, airway inflammation was exacerbated using dusty hay for 4 weeks. Clinical respiratory signs, rebreathing exam findings, and pulmonary inflammation were assessed.

Results

In asthmatic horses in Part One, BAL % neutrophils and BAL IL-6 concentrations significantly decreased post-supplementation (P = 0.02; 95 % CI = 93.86 to 913.2 pg/ml), and a greater decrease in BAL IL-10 was observed in asthmatic compared to healthy horses (P = 0.02; 95 % CI = -22.4 to -0.01). In Part Two, the proportion of horses with abnormal rebreathing exams differed significantly between groups (P = 0.002; 95 % CI = 0.11 to 0.41), and supplemented horses had significantly lower respiratory rates (P = 0.008; 95 % CI = -18.0 to -4.0).

Conclusions

Polyphenol supplementation may modulate pulmonary inflammation and improve clinical signs in mildly asthmatic horses when combined with environmental management.

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混合多酚对马哮喘综合征马下气道炎症的影响。

背景：马哮喘综合征（EAS）是成年马的一种慢性炎症性呼吸道疾病。虽然膳食多酚在人类哮喘中显示出抗炎作用，但它们在马哮喘中的应用尚未得到研究。目的：评价混合多酚补充剂对健康和哮喘马的全身和肺部炎症、支气管肺泡灌洗（BAL）细胞学和临床呼吸体征的影响。我们假设补充多酚可以减少健康和哮喘马的全身和肺部炎症，并改善哮喘马的BAL细胞学和临床呼吸体征。方法：在第一部分，18匹马（8匹健康马，10匹哮喘马）每天服用混合多酚补充剂，持续6-8周。补充前后进行体格检查、BAL细胞学检查以及血清和BAL液中细胞因子浓度的评估。第二部分，将10匹哮喘马随机分为补充组和对照组（n = 5匹/组）。在低粉尘饮食（含或不含补充）4周后，使用含灰尘干草4周，气道炎症加剧。评估临床呼吸体征、再呼吸检查结果和肺部炎症。结果：在第一部分的哮喘马中，BAL %中性粒细胞和BAL IL-6浓度在补充后显著降低（P = 0.02;95% CI = 93.86至913.2 pg/ml），并且与健康马相比，哮喘马的BAL IL-10降低幅度更大（P = 0.02;95% CI = -22.4至-0.01）。在第二部分中，再呼吸检查异常的马的比例在各组之间有显著差异（P = 0.002;95% CI = 0.11 ~ 0.41），补充马的呼吸率显著降低（P = 0.008;95% CI = -18.0 ~ -4.0）。结论：与环境管理相结合，多酚补充可调节轻度哮喘马的肺部炎症并改善临床症状。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Equine Veterinary Science 农林科学-兽医学

CiteScore

2.70

自引率

7.70%

发文量

249

审稿时长

77 days

期刊介绍： Journal of Equine Veterinary Science (JEVS) is an international publication designed for the practicing equine veterinarian, equine researcher, and other equine health care specialist. Published monthly, each issue of JEVS includes original research, reviews, case reports, short communications, and clinical techniques from leaders in the equine veterinary field, covering such topics as laminitis, reproduction, infectious disease, parasitology, behavior, podology, internal medicine, surgery and nutrition.