Biocompatibility of a Novel Light-Curable Hydrogel-Based Root Canal Obturation Material: In Vivo and In Vitro Analyses.

IF 3.6 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of endodontics Pub Date : 2025-08-28 DOI:10.1016/j.joen.2025.08.017

Moe Sandar Kyaw, Yoshio Yahata, Masato Nakano, Fusami Toyama, Chen Ke, Wang Shuai, Yuya Kamano, Futaba Harada, Tomose Noguchi, Masahiro Saito

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引用次数: 0

Abstract

Introduction: This study aims to evaluate the biocompatibility of a new light-curable hydrogel-based root canal obturation material, OdneFill, through in vivo and in vitro analyses.

Methods: Intentional overinstrumentation and overobturation were performed on the mandibular first molars of 10-week-old male rats, divided into 4 groups: group 1 (instrumentation only), group 2 (OdneFill), group 3 (gutta-percha + AH Plus), and group 4 (sound teeth). Mandibles were dissected after 3, 28, and 90 days for micro-computed tomography, histological analysis, and immunohistochemical staining and analyzed by two-way analysis of variance and post hoc Tukey test. Cytotoxicity and proinflammatory cytokine expression were assessed using RAW 264.7 cells and analyzed by 1-way analysis of variance and Tukey test (α = 0.05).

Results: Micro-computed tomographic analysis showed no significant difference in bone resorption (P > .05). However, AH Plus exhibited a higher inflammatory score (score: 1%-90%, score: 2%-10%) with increased neutrophil and macrophage infiltration in immunostaining (P < .05) compared to the instrumentation only group at day 90. In contrast, Odnefill showed comparable results (score: 0%-50%, score: 1%-30%, score: 2%-20%) to the instrumentation-only group (P > .05). Moreover, Odnefill did not affect the viability of RAW 264.7 cells, whereas the AH Plus extract decreased cell viability and upregulated inflammatory cytokines such as interleukin-1 beta and interleukin-6.

Conclusions: OdneFill demonstrated superior biocompatibility, and a minimal inflammatory response compared to AH Plus.

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一种新型光固化水凝胶根管封闭材料的生物相容性：体内和体外分析。

本研究旨在通过体内和体外分析来评估一种新型光固化水凝胶基根管封闭材料OdneFill（瑞士Odne）的生物相容性。方法：对10周龄雄性大鼠下颌第一磨牙进行故意过内固定和过封闭，分为4组：1组（仅内固定）、2组（OdneFill）、3组（古塔百尔沙+ AH Plus）和4组（健全牙）。分别于第3、28和90天解剖下颌骨，进行显微计算机断层扫描、组织学分析和免疫组织染色（IHC），并通过双向方差分析和事后Tukey检验进行分析。采用RAW 264.7细胞评价细胞毒性和促炎细胞因子的表达，采用单因素方差分析和Tukey检验（α = 0.05）。结果：微计算机层析分析显示骨吸收差异无统计学意义（P < 0.05）。然而，与仅使用器械组相比，AH Plus在第90天表现出更高的炎症评分（评分：1-90%，2-10%），免疫组化染色中中性粒细胞和巨噬细胞浸润增加（P < 0.05）。相比之下，Odnefill显示了与仅使用器械组相当的结果（评分：0-50%，1-30%,2-20%）（P < 0.05）。此外，Odnefill不影响RAW 264.7细胞的活力，而AH Plus提取物降低细胞活力并上调炎症细胞因子如IL-1β和IL-6。结论：与AH Plus相比，OdneFill表现出优越的生物相容性和最小的炎症反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of endodontics 医学-牙科与口腔外科

CiteScore

8.80

自引率

9.50%

发文量

224

审稿时长

42 days

期刊介绍： The Journal of Endodontics, the official journal of the American Association of Endodontists, publishes scientific articles, case reports and comparison studies evaluating materials and methods of pulp conservation and endodontic treatment. Endodontists and general dentists can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation in the one journal that helps them keep pace with rapid changes in this field.