Investigation of Endodontic Disinfection by Combination of Laser and Nanobubble Technology.

IF 3.6 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of endodontics Pub Date : 2025-08-30 DOI:10.1016/j.joen.2025.08.016

Haosu Ci, BingCheng Xin, Andrew W Hubbard, Evan Hamilton, Chaz Ainsworth, Enrique R Bursian, Yuting Zhang, Yanhui Zhang, Brian R Morrow, Misako Nakashima, George J-T Huang

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引用次数: 0

Abstract

Introduction: To investigate the combined use of laser, nanobubble water (NB-H₂O), and calcium hydroxide (Ca(OH)₂) for endodontic disinfection, particularly in regenerative endodontic cases where maximum effectiveness is needed.

Methods: Canal of human teeth were prepared, sterilized and inoculated with Enterococcus faecalis (E. faecalis). Teeth were assigned to 4 groups for two rounds of disinfection with Er,Cr: YSGG laser (Biolase). Group I - control, no disinfection, Group II - 6.25% NaOCl, Group III - 1.25% NaOCl, and Group IV - 1.25% NaOCl/NB-H₂O. Ca(OH)₂ was placed in the canal for ∼10 days between the two disinfection rounds. Teeth were evaluated by Scanning Electron Microscopy (SEM) for biofilm removal and Confocal Laser Scanning Microscopy (CLSM) to detect live/dead bacteria in the dentinal tubules.

Results: SEM analysis of the canal disinfection indicated that the NB-H₂O group (1.25% NaOCl/NB-H₂O) appeared to enhance the removal of biofilm/smear layer. However, it was not consistently more effective than other groups in killing the bacteria in the dentinal tubules. The combined use of low-concentration NaOCl, laser, NB-H₂O and Ca(OH)₂ largely reduced E. faecalis but did not achieve complete eradication from the canal or in the dentinal tubules.

Conclusion: Our comprehensive disinfection protocol reduced the bacteria in the dentinal tubules to a level potentially suited for regenerative endodontic therapy.

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激光与纳米气泡联合消毒牙髓的研究。

简介：研究激光、纳米泡水（NB-H2O）和氢氧化钙（Ca(OH)2）联合用于根管消毒，特别是在需要最大效果的再生根管病例中。方法：制备人牙管，消毒后接种粪肠球菌（E. faecalis）。采用Er、Cr: YSGG激光（Biolase）对牙齿进行2轮消毒。第一组为对照组，未消毒，第二组为6.25% NaOCl，第三组为1.25% NaOCl，第四组为1.25% NaOCl/NB-H2O。Ca(OH)2在两轮消毒之间放置在管中~ 10天。采用扫描电镜（SEM）检查生物膜的去除情况，用共聚焦激光扫描显微镜（CLSM）检查牙本质小管内的活/死细菌。结果：管道消毒后的扫描电镜分析表明，NB-H2O组（1.25% NaOCl/NB-H2O）对生物膜/涂抹层的去除有促进作用。然而，在杀死牙本质小管中的细菌方面，它并不总是比其他组更有效。低浓度NaOCl、激光、NB-H2O和Ca(OH)2联合使用可显著减少粪肠球菌，但不能完全根除根管或牙本质小管内的粪肠球菌。结论：我们的综合消毒方案将牙本质小管中的细菌减少到可能适合再生根管治疗的水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of endodontics 医学-牙科与口腔外科

CiteScore

8.80

自引率

9.50%

发文量

224

审稿时长

42 days

期刊介绍： The Journal of Endodontics, the official journal of the American Association of Endodontists, publishes scientific articles, case reports and comparison studies evaluating materials and methods of pulp conservation and endodontic treatment. Endodontists and general dentists can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation in the one journal that helps them keep pace with rapid changes in this field.