Genetic Neural Network-Assisted Natural Deep Eutectic Solvent Extraction for Enhanced Bioactive Yield from Folium Mori with Cytotoxicity Profiling.

IF 1.3 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of chromatographic science Pub Date : 2025-08-30 DOI:10.1093/chromsci/bmaf048

Yanxia Zhou, Jinglin Shen, Hongmei Chen, Ping Huang

引用次数: 0

Abstract

Natural deep eutectic solvents (NaDESs) was efficient and green for componential extraction of traditional Chinese medicine compared to conventional organic solvents. In this study, a total of nine NaDESs were synthesized and screened, and Proline-Urea (Pro-Ur) was screened as extract solvent for experiments. Five bioactive components (neochlorogenic acid, chlorogenic acid, rutin, isoquercitrin and kaempferol-3-O-rutinoside) were selected as target components. The optimal process was as follows: NaDES content of 60%, solid to liquid ratio of 80 mg/mL, extract temperature of 70°C and extract time of 40 min. The predicted comprehensive value of extraction yield was 0.4364. The IC50 of Betaine-Urea on PC12 cells was ~0.3% (v/v).

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遗传神经网络辅助天然深共熔溶剂萃取提高桑叶生物活性产率及细胞毒性分析。

与传统的有机溶剂相比，天然深共晶溶剂（NaDESs）是高效、绿色的中药成分提取溶剂。本研究共合成并筛选了9个NaDESs，并筛选了脯氨酸-尿素（Pro-Ur）作为提取溶剂进行实验。选择5种生物活性成分（新绿原酸、绿原酸、芦丁、异槲皮苷和山奈酚-3- o -芦丁苷）作为靶成分。最佳提取工艺为：NaDES含量60%，料液比80 mg/mL，提取温度70℃，提取时间40 min。预测提取率的综合值为0.4364。甜菜碱-尿素对PC12细胞的IC50为~0.3% （v/v）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of chromatographic science 化学-分析化学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

5.6 months

期刊介绍： The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.