Simultaneous Analysis of Cyproconazole and Tebuconazole in Suspension Concentrate Formulation by Reverse-Phase High-Performance Liquid Chromatography.

IF 1.3 4区 化学 Q4 BIOCHEMICAL RESEARCH METHODS
Sahadev Katam, Prasad Munipalli, Bhavani Balram, Vasu Govardhana Reddy Peddiahgari
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Abstract

A novel and validated reverse-phase high-performance liquid chromatography (RP-HPLC) approach was established for the concurrent measurement of cyproconazole (CYP) isomers and tebuconazole (TBZ) in suspension concentrate (SC) agrochemical formulations. The approach employed a C18 column with a gradient elution of 0.1% formic acid in water and methanol, attaining baseline resolution of CYP isomer-1, isomer-2 and TBZ without requiring chiral columns or sample pretreatment. Linearity was confirmed within the range of 80-120% of the target concentration, with a R2 value of 0.999. Accuracy varied from 101.2% to 102.8%, with a %RSD of less than 0.8%. LC-MS analysis verified analyte identity through distinctive ion transitions (CYP: m/z 292, TBZ: m/z 308). This is the initial method for isomer-specific detection of CYP using RP-HPLC within a formulation matrix. The methodology is straightforward, resilient and appropriate for standard quality assurance and regulatory adherence in agrochemical manufacturing.

反相高效液相色谱法同时分析悬浮液中环丙康唑和戊康唑的含量。
建立了一种新型高效液相色谱(RP-HPLC)方法,用于同时测定农药悬浊液浓缩物(SC)中环丙康唑(CYP)异构体和戊康唑(TBZ)的含量。该方法采用C18色谱柱,在水和甲醇中以0.1%甲酸梯度洗脱,无需手性色谱柱或样品预处理即可获得CYP异构体1、异构体2和TBZ的基线分辨率。在目标浓度80 ~ 120%范围内呈线性关系,R2为0.999。准确度在101.2% ~ 102.8%之间,%RSD小于0.8%。LC-MS分析通过不同的离子跃迁(CYP: m/z 292, TBZ: m/z 308)验证了分析物的身份。这是在配方基质中使用RP-HPLC对CYP进行异构体特异性检测的初始方法。该方法简单、有弹性,适用于农用化学品生产中的标准质量保证和法规遵守。
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来源期刊
CiteScore
2.90
自引率
7.70%
发文量
94
审稿时长
5.6 months
期刊介绍: The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.
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