Natural products eugenol and cinnamaldehyde as potential therapeutic alternatives against Candida auris infections.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Microbiology Pub Date : 2025-08-04 DOI:10.1093/jambio/lxaf197

Débora S M de Sousa, Gabrielle P M Monari, Cassiana F da Rosa, Jacques F Meis, Ana Flavia M Pereira, Tatiane B Zapata, Cauê B T Dos Santos, Mirian C E Aal, Flavia K Delella, Sandra M G Bosco, Ary F Júnior, Márcia S C Melhem, Eloisa F Clemente, Bruna B C Mendonça, Bruno C Rossini, Lucilene D Dos Santos, Luciana S Ruiz

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引用次数: 0

Abstract

Aims: Candida auris has been reported on all continents, becoming a global threat due to high levels of resistance to all antifungal classes. In addition, this pathogen has lower susceptibility to disinfectants used in hospitals, favoring its stay in such environments. Evaluate the action of eugenol and cinnamaldehyde against Candida auris.

Methods and results: The products were evaluated through sensitivity tests of broth microdilution, as well as their possible mechanisms of action using proteomic analysis. For microdilution was used 24 strains and for synergistic interactions was used 2 strains. Cytotoxicity of these products was verified in Vero cells. Eugenol and cinnamaldehyde showed fungicidal action at concentrations ranging from 31.25 to 250 µg/mL. Combinations of eugenol and cinnamaldehyde with itraconazole showed either antagonistic effects or no detectable interaction. No cytotoxicity was found for eugenol at the concentrations that caused yeast growth inhibition. On the other hand, cinnamaldehyde was cytotoxic at the same concentrations. According to proteomic analysis, the mechanism of action of eugenol and cinnamaldehyde is possibly related to the biosynthesis of ergosterol.

Conclusion: The present results suggest eugenol as a possible candidate for the development of formulations to treat infections caused by C. auris.

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天然产物丁香酚和肉桂醛作为抗耳念珠菌感染的潜在治疗选择。

目的：耳念珠菌已在各大洲报道，由于对所有抗真菌类的高水平耐药性而成为全球威胁。此外，这种病原体对医院使用的消毒剂的敏感性较低，有利于在这种环境中生存。丁香酚和肉桂醛对耳念珠菌的作用。方法与结果：通过微量肉汤稀释剂的敏感性试验对产物进行评价，并通过蛋白质组学分析对其可能的作用机制进行评价。微量稀释用24株，协同作用用2株。在Vero细胞中证实了这些产品的细胞毒性。丁香酚和肉桂醛在31.25 ~ 250µg/mL范围内均有杀真菌作用。丁香酚和肉桂醛与伊曲康唑联用显示拮抗作用或无检测到相互作用。丁香酚在引起酵母生长抑制的浓度下未发现细胞毒性。另一方面，肉桂醛在相同浓度下具有细胞毒性。根据蛋白质组学分析，丁香酚和肉桂醛的作用机制可能与麦角甾醇的生物合成有关。结论：丁香酚可作为治疗金黄色葡萄球菌感染的候选制剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.