The role of amino acid composition in the antibacterial activity of laterosporulin analogs.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI:10.1093/jambio/lxaf208

Piyush Baindara, Vipul Solanki, Shelley Sardul Singh, Nayudu Nallabelli, Sumeeta Kumari, Pushpanjali Bhardwaj, Vishakha Grover, Krishan Gopal Thakur, Suresh Korpole

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引用次数: 0

Abstract

Aim: This study aimed to compare the antimicrobial activity of the structural analogs of a class IId bacteriocin, laterosporulin.

Methods and results: The antimicrobial peptide laterosporulin3 (LS3), produced by a Brevibacillus sp. strain SKR3, showed 96% identity to earlier described laterosporulin (LS) and 60% to laterosporulin10 (LS10) in amino acid composition. LS3 has a molecular weight of 5613 Da, the same as LS, yet differs in amino acid composition. Notable differences were found in their antimicrobial activity. Crystal structures of LS3 and LS10 were compared with LS. The analysis revealed that an additional Lys residue in LS3 enhances its net positive charge, leading to increased antimicrobial activity. FITC labeling showed the role of surface-displayed cationic Lys residues in the activity.

Conclusion: Crystal structures of three different laterosporulins isolated from various strains of Brevibacillus were compared to understand the structure/function relationship. Results obtained in this study suggest that subtle variations in the amino acid composition of laterosporulins, distribution, and surface accessibility of charged residues can profoundly affect their antimicrobial activity.

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氨基酸组成对侧孢菌素类似物抑菌活性的影响。

目的：比较一类细菌素的结构类似物——后孢霉素的抑菌活性。方法与结果：由短芽孢杆菌菌株SKR3制备的抗菌肽laterosporulin3 （LS3）在氨基酸组成上与前人描述的laterosporulin （LS）的相似性为96%，与laterosporulin10 （LS10）的相似性为60%。LS3的分子量为5613 Da，与LS相同，但氨基酸组成不同。它们的抑菌活性存在显著差异。比较了LS3和LS10与LS的晶体结构。分析表明，LS3中添加的Lys残基增强了其净正电荷，从而提高了抗菌活性。FITC标记显示了表面显示的阳离子赖氨酸残基在活性中的作用。结论：比较了从不同短芽孢杆菌中分离的3种不同侧孢霉素的晶体结构，了解了其结构与功能的关系。本研究结果表明，侧孢菌素的氨基酸组成、分布和带电残基的表面可及性的细微变化会深刻影响其抗菌活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.