Impact of Sociodemographic, Clinical, and Genetic Factors and Fusobacterium nucleatum on Premature Birth Outcomes in Women from Kazakhstan: A Case-Control Study.

IF 1.5 Q2 MEDICINE, GENERAL & INTERNAL
Gulmira Zhurabekova, Aru Balmagambetova, Zhibek Oralkhan, Maygul Sarsenova, Guliya Toichiyeva, Nazym Altynbay, Azhar Malik, Kuanysh Tastambek, Akzhenis Berdalinova
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引用次数: 0

Abstract

Background: Premature birth (delivery before 37 weeks of gestation) is a leading cause of neonatal morbidity and mortality worldwide. Specific genetic polymorphisms were associated with immune and inflammatory pathways that might contribute to its pathogenesis. This study investigated the associations between preterm birth and sociodemographic indicators, clinical outcomes, genetic polymorphisms, and microbial factors in Kazakh women.

Methods: This case-control study was conducted from September 2022 to September 2023. It enrolled women with preterm and full-term births in Kyzylorda, Kazakhstan. Data included sociodemographic, clinical variables, genotyping (TLR2 rs4986790 and MBL2 rs11003125), and microbial data. The quantification of Fusobacterium nucleatum (F. nucleatum) in saliva samples was performed using TaqMan real-time PCR. Statistical analysis was conducted using SPSS software (version 26), employing independent sample t tests, Chi square tests, Mann-Whitney U tests, and logistic regression. The level of significance was set at P<0.05.

Results: Sociodemographic analysis showed no significant differences between the studied groups, except for a higher prevalence of previous preterm birth in the case group. Clinical comparisons revealed significantly lower gestational age at delivery, reduced newborn weight and height, decreased placental weight and dimensions, lower hemoglobin levels, and erythrocyte counts in preterm cases. Genetic analysis demonstrated that all women with preterm labor carried the homozygous AA genotype of TLR299 rs4986790, while the GG genotype and the G allele of the MBL2 rs11003125 gene were predominant in this group. Furthermore, the quantitative analysis identified significantly higher F. nucleatum levels associated with premature birth, highlighting a potential microbial role in its pathogenesis.

Conclusion: These findings suggested that a history of preterm birth, specific genetic polymorphisms, and microbial factors collectively were associated with an increased risk of preterm birth.

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社会人口学、临床和遗传因素以及核梭杆菌对哈萨克斯坦妇女早产结局的影响:一项病例对照研究。
背景:早产(妊娠37周前分娩)是全世界新生儿发病率和死亡率的主要原因。特定的遗传多态性与免疫和炎症途径相关,可能有助于其发病机制。本研究调查了哈萨克妇女早产与社会人口指标、临床结果、遗传多态性和微生物因素之间的关系。方法:本研究于2022年9月至2023年9月进行病例对照研究。它招募了哈萨克斯坦Kyzylorda的早产和足月分娩妇女。数据包括社会人口学、临床变量、基因分型(TLR2 rs4986790和MBL2 rs11003125)和微生物数据。采用TaqMan实时荧光定量PCR技术对唾液样品中具核梭杆菌(F. nucleatum)进行定量分析。统计学分析采用SPSS软件(version 26),采用独立样本t检验、卡方检验、Mann-Whitney U检验和logistic回归。结果:社会人口统计学分析显示,除了病例组中先前早产的患病率较高外,各组之间没有显著差异。临床比较显示,早产病例的分娩胎龄明显降低,新生儿体重和身高降低,胎盘重量和尺寸降低,血红蛋白水平和红细胞计数降低。遗传分析表明,所有早产妇女均携带TLR299 rs4986790纯合AA基因型,而MBL2 rs11003125基因的GG基因型和G等位基因在该组中占主导地位。此外,定量分析发现,较高的核梭菌水平与早产有关,强调了其发病机制中潜在的微生物作用。结论:这些发现表明,早产史、特定遗传多态性和微生物因素共同与早产风险增加有关。
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来源期刊
Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
3.20
自引率
0.00%
发文量
84
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Medical Sciences (IJMS) is an international quarterly biomedical publication, which is sponsored by Shiraz University of Medical Sciences. The IJMS intends to provide a scientific medium of com­muni­cation for researchers throughout the globe. The journal welcomes original clinical articles as well as clinically oriented basic science re­search experiences on prevalent diseases in the region and analysis of various regional problems.
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