The expression trend and regulatory role of miR-484 in patients with gestational diabetes mellitus.

Abstract

Objective: Gestational diabetes mellitus (GDM) is characterized by the onset of abnormal glucose metabolism during pregnancy. This study aims to examine the expression patterns and functional significance of miR-484 in GDM.

Methods: The levels of miR-484 in GDM patients were compared to those in normal pregnant women through RT-qPCR. Subsequently, the diagnostic and predictive capacity of miR-484 for GDM was assessed utilizing Receiver Operating Characteristic (ROC) curve. In the next phase, the effects of miR-484 on blood glucose levels and insulin resistance in GDM mice were studied by establishing a GDM mouse model. Finally, pancreatic tissues were isolated to elucidate the influence of miR-484 on inflammation processes and oxidative stress.

Results: Elevated levels of miR-484 have been observed in GDM patients, demonstrating a positive correlation with blood glucose levels. ROC curve analysis indicated that miR-484 possesses substantial diagnostic and predictive capabilities for GDM, with an AUC of 0.835, a sensitivity of 80.95%, and a specificity of 82.00%. Furthermore, in GDM models, the downregulation of miR-484 significantly mitigated the adverse effects associated with GDM, leading to notable reductions in blood glucose levels and insulin resistance. Following the down-regulation of miR-484, a notable reduction in the levels of IL-1β, TNF-α, IL-6, and MDA in the pancreas was also observed. Concurrently, there was a significant increase in the levels of SOD and CAT.

Conclusion: miR-484 might act as a biomarker for the early-stage recognition of GDM. Down-regulating this molecule is beneficial for lowering blood glucose, insulin resistance, and pancreatic inflammation or oxidative stress.