Variovorax arabinosiphilus sp. nov., Variovorax flavidus sp. nov., Variovorax gracilis sp. nov., Variovorax brevis sp. nov., Variovorax jilinensis sp. nov., Variovorax davisae sp. nov., Variovorax saccharolyticus sp. nov. and Variovorax fucosicus sp. nov., isolated from ginseng rhizosphere.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-08-01 DOI:10.1099/ijsem.0.006895

Yu-Hang Jiang, Ce-Ce Yin, Lei-Lei Yang, Yu-Hua Xin, Qing Liu, Jian Ye

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Abstract

Thirteen novel strains - J2L1-78^T, J2P1-59^T, J22R24^T, J22R133^T, J22P168^T, J22P271^T, J22R187^T, J22R193^T, J2L1-63, J2R1-6, J22G21, J22G47, and J31P216 - were isolated from the rhizosphere of 20-year-old ginseng in Jilin Province, P.R. China. These strains were Gram-stain-negative, aerobic and rod-shaped. Phylogenetic analysis based on the 16S rRNA gene sequences indicated their affiliation to the genus Variovorax, with 98.8-99.6% sequence similarity to Variovorax ginsengisoli Gsoil 3165^T, Variovorax ureilyticus UCM-2^T and Variovorax humicola UC38^T. Phylogenomic analysis demonstrated their distinctiveness from closely related species. Average nucleotide identity (ANI) values among these strains confirm their representation of eight distinct species; additionally, ANI and digital DNA-DNA hybridization values between these strains and their closest relatives were below 92.5 and 54.1%, respectively. Eight representative strains contained C_16 : 0, summed feature 3 (C_16 : 1 ω7c and/or C_16 : 1 ω6c) and C_17 : 0 cyclo as major fatty acids. Based on phenotypic, phylogenetic and genotypic data, these 13 strains comprise 8 novel species within the genus Variovorax, for which we propose the following names: Variovorax arabinosiphilus sp. nov. (J2L1-78^T=CGMCC 1.60704^T=KACC 23365^T), Variovorax flavidus sp. nov. (J2P1-59^T=CGMCC 1.60707^T=KACC 23366^T), Variovorax gracilis sp. nov. (J22R24^T=CGMCC 1.61001^T=KACC 23367^T), Variovorax brevis sp. nov. (J22R133^T=CGMCC 1.61263^T=KACC 23368^T), Variovorax jilinensis sp. nov. (J22P168^T=CGMCC 1.64555^T=KACC 23372^T), Variovorax davisae sp. nov. (J22P271^T=CGMCC 1.64593^T=KACC 23373^T), Variovorax saccharolyticus sp. nov. (J22R187^T=CGMCC 1.64629^T=KACC 23374^T) and Variovorax fucosicus sp. nov. (J22R193^T=CGMCC 1.64631^T=KACC 23375^T).

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从人参根际分离的阿拉伯型变异螨、黄型变异螨、薄厚型变异螨、短型变异螨、吉林型变异螨、davisae型变异螨、糖溶型变异螨和焦点型变异螨。

从吉林省20年人参根际分离到13株新菌株J2L1-78T、J2P1-59T、J22R24T、J22R133T、J22P168T、J22P271T、J22R187T、J22R193T、J2L1-63、J2R1-6、J22G21、J22G47和J31P216。这些菌株革兰氏染色阴性，好氧，杆状。基于16S rRNA基因序列的系统发育分析表明，该菌株属于Variovorax ginsengisoli Gsoil 3165T、Variovorax uilyticus UCM-2T和Variovorax humicola UC38T，序列相似性为98.8% ~ 99.6%。系统基因组学分析表明，它们与近缘种有明显区别。这些菌株的平均核苷酸同一性（ANI）值证实它们代表八个不同的物种；与近亲属的ANI和数字dna杂交值分别低于92.5%和54.1%。8株代表性菌株的主要脂肪酸为C16: 0、特征3 （C16: 1 ω7c和/或C16: 1 ω6c）和C17: 0环。根据表型、系统发育和基因型数据，这13个菌株包括8个Variovorax属的新种，我们提出以下名称：11月：arabinosiphilus varovorax sp. 11 （j2p1 - 78t =CGMCC 1.60704T=KACC 23365T）、11月：Variovorax flavidus sp. 11 （J2P1-59T=CGMCC 1.60707T=KACC 23366T）、11月：Variovorax gracilis sp. 11 （J22R24T=CGMCC 1.661001 t =KACC 23367T）、11月：Variovorax brevis sp. 11 （J22R133T=CGMCC 1.61263T=KACC 23368T）、11月：Variovorax jiilinensis sp. 11 （J22P168T=CGMCC 1.64555T=KACC 23372T）、11月：Variovorax davisae sp. 11 （J22P271T=CGMCC 1.64593T=KACC 23373T）Variovorax saccharolyticus sp. nov （J22R187T=CGMCC 1.64629T=KACC 23374T）和Variovorax focusicus sp. nov （J22R193T=CGMCC 1.64631T=KACC 23375T）。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.