Expression of the genes QPRT, RAB26, and SRPRB in cancer tissue from patients with triple-negative breast cancer as a biomarker for diagnosis, pharmacotherapy strategy, and survival: Evidence from bioinformatic database analysis .
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引用次数: 0
Abstract
Objective: To screen differentially expressed genes (DEGs) in triple-negative breast cancer (TNBC) by bioinformatic methods and explore their relationship between TNBC prognosis and related biological functions.
Materials and methods: Microarray dataset GSE65194 was downloaded from Gene Expression Omnibus (GEO) database. The whole TNBC-related datasets were downloaded from The Cancer Genome Atlas (TCGA) database. After screening DEGs in R software, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to predict the function of these DEGs. The survival curve examination of the DEGs was performed based on TCGA database and Kaplan-Meier plotter website. In addition, the gene-drug interaction network was explored using the Comparative Toxicogenomics Database (CTD).
Results: A total of 2,496 up-regulated and 142 down-regulated DEGs were revealed in GEO database and TCGA database simultaneously. The GO and KEGG analysis revealed enrichment in regulation of nucleobase, nucleoside, nucleic acid metabolism, DNA binding, and integrin family cell surface interactions. Furthermore, up-regulation of quinolinate phosphoribosyl transferase (QPRT), member RAS oncogene family (RAB26), and SRP receptor subunit beta (SRPRB) were significantly associated with survival in TNBC patients.
Conclusion: QPRT, RAB26, and SRPRB may be three potential novel prognostic biomarkers for TNBC and will provide potential guidance value for future research on TNBC.
目的：应用生物信息学方法筛选三阴性乳腺癌（TNBC）中差异表达基因（DEGs），探讨其与TNBC预后及相关生物学功能的关系。材料和方法：从Gene Expression Omnibus （GEO）数据库下载微阵列数据集GSE65194。从癌症基因组图谱（TCGA）数据库下载tnbc相关的全部数据集。在R软件中筛选deg后，进行基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析，预测这些deg的功能。基于TCGA数据库和Kaplan-Meier绘图仪网站对deg进行生存曲线检查。此外，利用比较毒物基因组学数据库（CTD）探索基因-药物相互作用网络。结果：在GEO数据库和TCGA数据库中同时发现了2496个上调的deg和142个下调的deg。GO和KEGG分析显示，核碱基、核苷、核酸代谢、DNA结合和整合素家族细胞表面相互作用的调控富集。此外，喹啉酸磷酸核糖基转移酶（QPRT）、RAS癌基因家族成员（RAB26）和SRP受体亚单位β (SRPRB)的上调与TNBC患者的生存显著相关。结论：QPRT、RAB26和SRPRB可能是三种潜在的新型TNBC预后生物标志物，对未来TNBC的研究具有潜在的指导价值。
期刊介绍：
The International Journal of Clinical Pharmacology and Therapeutics appears monthly and publishes manuscripts containing original material with emphasis on the following topics: Clinical trials, Pharmacoepidemiology - Pharmacovigilance, Pharmacodynamics, Drug disposition and Pharmacokinetics, Quality assurance, Pharmacogenetics, Biotechnological drugs such as cytokines and recombinant antibiotics. Case reports on adverse reactions are also of interest.
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