Porphyromonas gingivalis and Fusobacterium nucleatum synergistically strengthen the effect of promoting oral squamous cell carcinoma progression.

IF 2.8 2区医学 Q3 IMMUNOLOGY

Infectious Agents and Cancer Pub Date : 2025-08-29 DOI:10.1186/s13027-025-00689-5

Xiao Song, Jingfei Wang, Zhen Gu, Xinyi Qiu, Meng Yuan, Huiji Ke, Runzhi Deng

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引用次数: 0

Abstract

Objective: To investigate the mechanism of action of Porphyromonas gingivalis (P. gingivalis) and Fusobacterium nucleatum (F. nucleatum) individually and synergistically on Cal-27 cells through transcriptome analyses to evaluate the mechanism evidence of periodontal pathogen involvement in oral squamous cell carcinoma.

Methods: Cal-27 cells were treated with P. gingivalis and F. nucleatum individually or in combination. Cell proliferation was assessed via CCK-8 assay and EdU staining, while migration was evaluated using scratch assays. Transcriptomic sequencing analyzed molecular mechanisms underlying single and co-infections.

Results: Synergistic treatment with P. gingivalis and F. nucleatum significantly enhanced Cal-27 cell proliferation and migration compared to either pathogen alone. Transcriptomics revealed that co-infection accelerated tumor cell cycle progression and amplified pro-inflammatory pathways, indicating stronger pro-tumorigenic effects.

Conclusion: This study clarifies the cooperative tumor-promoting role of multiple bacterial species, providing potential therapeutic targets for oral squamous cell carcinoma in bacterial infection contexts and highlighting the importance of controlling oral microbiota.

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牙龈卟啉单胞菌和核梭杆菌协同增强促进口腔鳞状细胞癌进展的作用。

目的：通过转录组分析，探讨牙龈卟啉单胞菌（P. gingivalis）和核梭杆菌（F. nucleatum）单独和协同作用于Cal-27细胞的机制，探讨牙周病原体参与口腔鳞状细胞癌的机制证据。方法：分别用牙龈假单胞菌和具核假单胞菌分别或联合作用于Cal-27细胞。通过CCK-8法和EdU染色评估细胞增殖，通过划痕法评估细胞迁移。转录组测序分析了单一感染和合并感染的分子机制。结果：牙龈假单胞菌和具核假单胞菌协同作用可显著增强Cal-27细胞的增殖和迁移能力。转录组学显示，联合感染加速了肿瘤细胞周期进程，放大了促炎途径，表明更强的促肿瘤作用。结论：本研究阐明了多种细菌的协同促瘤作用，为细菌感染下口腔鳞状细胞癌提供了潜在的治疗靶点，强调了控制口腔微生物群的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infectious Agents and Cancer ONCOLOGY-IMMUNOLOGY

CiteScore

5.80

自引率

2.70%

发文量

期刊介绍： Infectious Agents and Cancer is an open access, peer-reviewed online journal that encompasses all aspects of basic, clinical, epidemiological and translational research providing an insight into the association between chronic infections and cancer. The journal welcomes submissions in the pathogen-related cancer areas and other related topics, in particular: • HPV and anogenital cancers, as well as head and neck cancers; • EBV and Burkitt lymphoma; • HCV/HBV and hepatocellular carcinoma as well as lymphoproliferative diseases; • HHV8 and Kaposi sarcoma; • HTLV and leukemia; • Cancers in Low- and Middle-income countries. The link between infection and cancer has become well established over the past 50 years, and infection-associated cancer contribute up to 16% of cancers in developed countries and 33% in less developed countries. Preventive vaccines have been developed for only two cancer-causing viruses, highlighting both the opportunity to prevent infection-associated cancers by vaccination and the gaps that remain before vaccines can be developed for other cancer-causing agents. These gaps are due to incomplete understanding of the basic biology, natural history, epidemiology of many of the pathogens that cause cancer, the mechanisms they exploit to cause cancer, and how to interrupt progression to cancer in human populations. Early diagnosis or identification of lesions at high risk of progression represent the current most critical research area of the field supported by recent advances in genomics and proteomics technologies.