Xueming Yao, Qiuyi Wang, Xuemei Yuan, Feng Luo, Yi Ling, Changming Chen, Wukai Ma, Zhu Yang
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引用次数: 0
Abstract
Aim: This study investigated the mechanisms by which 6-gingerol affected miRNAs in mesenchymal stem cell exosomes and the regulation on NF-κB signaling pathways in synovial cells and GES-1 via miR-30c-2-3p. In addition, the study evaluated potential protective effects of 6-gingerol on synovial tissue and gastric mucosa based on a rat model.
Methods: The role of 6-gingerol was evaluated using assays including Western Blotting, transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), qPCR, immunofluorescence, dual luciferase reporter gene assay, ELISA, CCK-8 cell viability assay, and protein expression analysis. Meanwhile, the effects of 6-gingerol on synovial tissue and gastric mucosa were evaluated using an animal model.
Results: 6-Gingerol exhibited anti-inflammatory effects by upregulating miR-30c-2-3p, targeting the TAB1 gene, and suppressing NF-κB signaling pathway activation. In both synovial cells and GES-1, 6-gingerol significantly reduced the concentration of inflammatory factors and increased cell viability. In addition, 6-gingerol regulated key molecules in the NF-κB signaling pathway through miR-30c-2-3p, thereby reducing inflammatory response. Based on the rat model, 6-gingerol showed protective effects on synovial tissue and gastric mucosa, while miR-30c-2-3p antagomir might attenuate the therapeutic effects.
Conclusion: 6-Gingerol effectively suppressed the NF-κB signaling pathway activation via upregulating miR-30c-2-3p expression, which targeted TAB1. This led to significant anti-inflammatory and protective effects in synovial cells and GES-1.
期刊介绍:
Inflammopharmacology is the official publication of the Gastrointestinal Section of the International Union of Basic and Clinical Pharmacology (IUPHAR) and the Hungarian Experimental and Clinical Pharmacology Society (HECPS). Inflammopharmacology publishes papers on all aspects of inflammation and its pharmacological control emphasizing comparisons of (a) different inflammatory states, and (b) the actions, therapeutic efficacy and safety of drugs employed in the treatment of inflammatory conditions. The comparative aspects of the types of inflammatory conditions include gastrointestinal disease (e.g. ulcerative colitis, Crohn''s disease), parasitic diseases, toxicological manifestations of the effects of drugs and environmental agents, arthritic conditions, and inflammatory effects of injury or aging on skeletal muscle. The journal has seven main interest areas:
-Drug-Disease Interactions - Conditional Pharmacology - i.e. where the condition (disease or stress state) influences the therapeutic response and side (adverse) effects from anti-inflammatory drugs. Mechanisms of drug-disease and drug disease interactions and the role of different stress states
-Rheumatology - particular emphasis on methods of measurement of clinical response effects of new agents, adverse effects from anti-rheumatic drugs
-Gastroenterology - with particular emphasis on animal and human models, mechanisms of mucosal inflammation and ulceration and effects of novel and established anti-ulcer, anti-inflammatory agents, or antiparasitic agents
-Neuro-Inflammation and Pain - model systems, pharmacology of new analgesic agents and mechanisms of neuro-inflammation and pain
-Novel drugs, natural products and nutraceuticals - and their effects on inflammatory processes, especially where there are indications of novel modes action compared with conventional drugs e.g. NSAIDs
-Muscle-immune interactions during inflammation [...]