The diagnostic and prognostic value of LncRNA FAM3D-AS1 expression in nasopharyngeal carcinoma and its effect on tumor progression and cisplatin resistance.

IF 2.5 3区生物学

Hereditas Pub Date : 2025-08-26 DOI:10.1186/s41065-025-00526-0

Bohan Zhang, Zixiao Chen, Zhinong Wu

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Abstract

Background: The location of nasopharyngeal carcinoma (NPC) is relatively hidden. Most patients are diagnosed at the middle or late stage of the disease, having missed the best time for treatment.

Aim: To explore the clinical value of lncRNA FAM3D-AS1 and its effect on tumor progression and cisplatin resistance in nasopharyngeal cancer.

Materials and methods: In this study, a total of 118 NPC patients and 50 healthy volunteers participated. RT-qPCR was analyzed to detect the lncRNA FAM3D-AS1 expression in NPC patients and cells. Receiver Operating Characteristic (ROC curves), Kaplan-Meier (K-M) survival curves and Cox regression analysis were performed to analyze the clinical value value of lncRNA FAM3D-AS1. Cell functional assays were employed to investigate the impact of lncRNA FAM3D-AS1 on biological malignant behavior of NPC and cisplatin resistance.

Results: In NPC patients' serum and cell lines, the expression of lncRNA FAM3D-AS1 was upregulated. ROC curves with Area Under the Curve (AUC) = 0.920, 95%CI = 0.874-0.965 could be used to distinguish the healthy group from the NPC group. K-M survival curves revealed that NPC patients with high expression of lncRNA FAM3D-AS1 had poor prognosis. Cox regression analysis suggested that lncRNA FAM3D-AS1 was an independent prognostic marker for NPC. Knockdown of lncRNA FAM3D-AS1 expression prohibited NPC cell proliferation, migration, invasion, and cisplatin resistance, but promoted apoptosis.

Conclusion: LncRNA FAM3D-AS1 can be used as a biomarker to diagnose and prognosticate NPC. LncRNA FAM3D-AS1 affects tumor progression and cisplatin resistance in NPC.

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LncRNA FAM3D-AS1表达在鼻咽癌中的诊断和预后价值及其对肿瘤进展和顺铂耐药的影响

背景：鼻咽癌（NPC）的发病部位相对隐蔽。大多数患者在疾病的中晚期被诊断出来，错过了治疗的最佳时机。目的：探讨lncRNA FAM3D-AS1在鼻咽癌中的临床价值及其对肿瘤进展和顺铂耐药的影响。材料与方法：本研究共纳入118例鼻咽癌患者和50名健康志愿者。RT-qPCR检测lncRNA FAM3D-AS1在鼻咽癌患者及细胞中的表达。采用受试者工作特征（ROC曲线）、Kaplan-Meier （K-M）生存曲线和Cox回归分析lncRNA FAM3D-AS1的临床价值。采用细胞功能检测研究lncRNA FAM3D-AS1对鼻咽癌生物学恶性行为及顺铂耐药的影响。结果：在鼻咽癌患者血清和细胞系中，lncRNA FAM3D-AS1表达上调。采用曲线下面积(AUC) = 0.920, 95%CI = 0.874 ~ 0.965的ROC曲线可以区分健康组和鼻咽癌组。K-M生存曲线显示lncRNA FAM3D-AS1高表达的鼻咽癌患者预后较差。Cox回归分析提示lncRNA FAM3D-AS1是鼻咽癌的独立预后指标。敲低lncRNA FAM3D-AS1表达抑制鼻咽癌细胞增殖、迁移、侵袭和顺铂耐药，但促进细胞凋亡。结论：LncRNA FAM3D-AS1可作为鼻咽癌诊断和预后的生物标志物。LncRNA FAM3D-AS1影响鼻咽癌肿瘤进展和顺铂耐药。

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来源期刊

Hereditas Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.80

自引率

3.70%

发文量

期刊介绍： For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.