Mycotoxin binder for immune and intestinal histopathology ameliorations against Newcastle disease in vaccinated broilers.

Q2 Pharmacology, Toxicology and Pharmaceutics

F1000Research Pub Date : 2025-07-25 eCollection Date: 2024-01-01 DOI:10.12688/f1000research.158103.3

Erma Safitri, Hery Purnobasuki, Tita Damayanti Lestari, Suzanita Utama, Merisa Wahyu Erdhina, Maulida Ilma Sadida, Eka Pramyrtha Hestianah, Mohammad Anam Al Arif, Chairul Anwar Nidom, Sri Mulyati, Jola Rahmahani, Martia Rani Tacharina, Sri Hidanah, Siti Darodjah Rasad, Goo Jang, Mitsuhiro Takagi, Muhammad Thohawi Elziyad Purnama

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Abstract

Background: In broiler farming, vaccination against Newcastle disease (ND) is essential. Nonetheless, during the post-vaccination phase, production may be negatively impacted by mycotoxin contamination in feed. This study aimed to evaluate the effect of mycotoxin binders on immune and intestinal histopathology ameliorations against ND in vaccinated broilers with aflatoxin B1 (AFB1) and ochratoxin A (OTA) toxication.

Methods: A total of 20 broilers were randomly assigned into 4 groups with 5 replications i.e. (C-) broiler groups with basal feed, (C+) broiler groups with AFB1 and OTA feed contamination, (T1) and (T2) broiler groups with exposed AFB1, OTA, and toxin binders as feed additives with dose 1.1 g/kg and 1.6 g/kg feed, respectively. ND vaccination was carried out on day 7 and 21. Antibody titers were evaluated from serum samples of broiler on days 14, 28, and 35 for further hemagglutination inhibition (HI) test. Histopathology of the cecum and colon organs was evaluated using HE staining on day 36. HI test and histological scoring were analyzed using the One-Way ANOVA, followed by Duncan's test with a p < 0.05 in SPSS v.26 software (IBM Corp., Armonk, NY).

Results: As a result, histopathological improvement of the cecum and colon was reported based on mucosal rupture, hemorrhage and necrosis on day 35. A significant increase in antibody titer was observed on day 35 in the C- and T2 groups, which differed significantly (p < 0.05) from the C+ and T1 groups.

Conclusions: This study revealed that a 1.6 g/kg toxin binder dose in feed can increase antibody titer and histopathology of cecum and colon in broiler chickens after ND vaccination fed with mycotoxin-contaminated feed.

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查看原文本刊更多论文

霉菌毒素结合剂对接种肉鸡新城疫免疫和肠道组织病理学的改善。

背景：在肉鸡养殖中，预防新城疫（ND）的接种是必不可少的。尽管如此，在接种疫苗后阶段，饲料中的霉菌毒素污染可能会对生产产生负面影响。本研究旨在评价霉菌毒素结合剂对黄曲霉毒素B1 （AFB1）和赭曲霉毒素A （OTA）接种肉鸡抗ND免疫和肠道组织病理学的改善作用。方法：将20只肉鸡随机分为4组，每组5个重复，分别为基础饲料（C-）组、AFB1和OTA饲料污染（C+）组、AFB1、OTA和毒素结合剂饲料暴露（T1）组和（T2）组，剂量分别为1.1 g/kg和1.6 g/kg。在第7天和第21天接种ND疫苗。分别在第14、28和35天对肉鸡血清样品进行抗体效价测定，以进行进一步的血凝抑制试验。第36天用HE染色评价盲肠和结肠器官的组织病理学。HI检验和组织学评分采用单因素方差分析，采用SPSS v.26软件（IBM Corp., Armonk， NY）进行Duncan检验，p < 0.05。结果：在第35天，盲肠和结肠粘膜破裂、出血和坏死的基础上报告了组织病理学改善。抗体滴度在第35天C-和T2组显著升高，与C+和T1组相比差异显著（p < 0.05）。结论：饲料中添加1.6 g/kg的毒素结合剂可提高肉鸡接种ND后盲肠和结肠的抗体效价和组织病理学。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

F1000Research Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

5.00

自引率

0.00%

发文量

1646

审稿时长

1 weeks

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