Immunohistochemical study of transient receptor potential Vanilloid (TRPV1-4) channels expression in human and rat corneas

IF 2.7 2区医学 Q1 OPHTHALMOLOGY

Experimental eye research Pub Date : 2025-08-29 DOI:10.1016/j.exer.2025.110616

Akepogu Jacquelyn , Bokara Kiran Kumar , Sirisha Senthil , Charanya Ramachandran

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引用次数: 0

Abstract

Transient Receptor potential Vanilloid (TRPV) channels are a group of cation channels that are expressed in a wide variety of tissues and are involved in regulating several cellular processes. An alteration in their expression and cellular localization is associated with various pathologies. In this study, we investigated the expression and localization of TRPV1-4 channels in human and rat corneas. TRPV 1–4 channels were found to be expressed in the epithelium, keratocytes, and endothelium of both species, which was confirmed at the gene and protein levels through PCR analysis and immunostaining. The channels were seen to be largely distributed at the cell membrane and cytoplasm, but the intensity of staining, quantified using Image J software, varied within and between cell layers. In addition, we found nuclear expression of these channels in corneal cells, and the same was quantified using ImageJ software. In both species, robust expression of TRPV1-4 channels, including in the cell nuclei, was noted in the epithelium and endothelium, compared to the stromal cells. This study provides evidence for the expression of TRPV2 and 3 channels, for which there is limited information in the corneal cells. In conclusion, this study confirms the expression of TRPV1-4 channels in the corneal cells of both species.

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瞬时受体电位香草蛋白（TRPV1-4）通道在人和大鼠角膜表达的免疫组织化学研究。

瞬时受体电位香草蛋白（TRPV）通道是一组阳离子通道，在多种组织中表达，并参与调节多种细胞过程。它们的表达和细胞定位的改变与各种病理有关。在本研究中，我们研究了TRPV1-4通道在人和大鼠角膜中的表达和定位。TRPV - 1-4通道在两种动物的上皮、角化细胞和内皮中均有表达，通过PCR分析和免疫染色在基因和蛋白水平上证实了这一点。可见通道主要分布在细胞膜和细胞质上，但使用Image J软件定量的染色强度在细胞层内和层间变化。此外，我们在角膜细胞中发现了这些通道的核表达，并使用ImageJ软件对其进行了量化。在这两个物种中，与基质细胞相比，在上皮细胞和内皮细胞中发现了TRPV1-4通道的强烈表达，包括细胞核。本研究为TRPV2和3通道的表达提供了证据，而TRPV2和3通道在角膜细胞中的表达信息有限。综上所述，本研究证实了TRPV1-4通道在两种物种角膜细胞中的表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental eye research 医学-眼科学

CiteScore

6.80

自引率

5.90%

发文量

323

审稿时长

66 days

期刊介绍： The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.