Expression characteristics of dual-specificity phosphatase 2 and hypoxia-inducible factor-1α in acute kidney injury and preliminary study of the effect of dual-specificity phosphatase 2 on HK-2 cells.

IF 2.8 4区 医学 Q2 PHYSIOLOGY
Xueqian Chu, Songmin Xue, Lanyan Lin, Xuan Huang, Sisi Chen, Wulaer Adeli, Zulibiya Tuohetiyaer, Suhua Li, Chen Lu
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引用次数: 0

Abstract

Acute kidney injury (AKI) is a global health problem with significant long-term harm if the prognosis is poor. Dual-specificity phosphatase 2 (DUSP2) is involved in key regulatory pathways in several disease processes, but its function in renal pathophysiology is unclear. The expression levels of DUSP2 in the peripheral blood of AKI and non-AKI patients were first examined. The expression characteristics of DUSP2 and hypoxia-inducible factor-1α (HIF-1α) in AKI conditions were analysed in renal tissues from AKI patients and different AKI model mice. Human renal tubular epithelial cells (HK-2) were utilized for in vitro experiments to investigate the effects of overexpression of DUSP2 on cell proliferation, apoptosis and HIF-1α levels under hypoxia-reoxygenation conditions. DUSP2 showed low expression in the peripheral blood serum of AKI patients. In the renal tissues of patients and mouse models of AKI, DUSP2 expression was significantly lower, and HIF-1α expression was significantly higher. Under hypoxia-reoxygenation conditions, DUSP2 overexpression promoted HK-2 cell proliferation, inhibited apoptosis and suppressed HIF-1α expression. The present study demonstrated a significant correlation between DUSP2 and HIF-1α expression in the context of AKI and revealed the protective effect of DUSP2 overexpression on renal tubular epithelial cells under hypoxia-reoxygenation, which provides a new better understanding of AKI.

双特异性磷酸酶2和缺氧诱导因子-1α在急性肾损伤中的表达特征及双特异性磷酸酶2对HK-2细胞影响的初步研究
急性肾损伤(AKI)是一个全球性的健康问题,如果预后不良,会造成严重的长期危害。双特异性磷酸酶2 (DUSP2)参与多种疾病过程的关键调控通路,但其在肾脏病理生理中的功能尚不清楚。首先检测AKI和非AKI患者外周血中DUSP2的表达水平。分析AKI患者和不同AKI模型小鼠肾组织中DUSP2和缺氧诱导因子-1α (HIF-1α)在AKI条件下的表达特征。利用人肾小管上皮细胞(HK-2)进行体外实验,探讨缺氧-复氧条件下DUSP2过表达对细胞增殖、凋亡及HIF-1α水平的影响。DUSP2在AKI患者外周血血清中低表达。在AKI患者和小鼠模型肾组织中,DUSP2表达显著降低,HIF-1α表达显著升高。缺氧复氧条件下,DUSP2过表达促进HK-2细胞增殖,抑制细胞凋亡,抑制HIF-1α表达。本研究证实了AKI背景下DUSP2与HIF-1α表达之间的显著相关性,揭示了DUSP2过表达对缺氧-再氧化肾小管上皮细胞的保护作用,为AKI提供了新的认识。
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来源期刊
Experimental Physiology
Experimental Physiology 医学-生理学
CiteScore
5.10
自引率
3.70%
发文量
262
审稿时长
1 months
期刊介绍: Experimental Physiology publishes research papers that report novel insights into homeostatic and adaptive responses in health, as well as those that further our understanding of pathophysiological mechanisms in disease. We encourage papers that embrace the journal’s orientation of translation and integration, including studies of the adaptive responses to exercise, acute and chronic environmental stressors, growth and aging, and diseases where integrative homeostatic mechanisms play a key role in the response to and evolution of the disease process. Examples of such diseases include hypertension, heart failure, hypoxic lung disease, endocrine and neurological disorders. We are also keen to publish research that has a translational aspect or clinical application. Comparative physiology work that can be applied to aid the understanding human physiology is also encouraged. Manuscripts that report the use of bioinformatic, genomic, molecular, proteomic and cellular techniques to provide novel insights into integrative physiological and pathophysiological mechanisms are welcomed.
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