Mechanism Exploration of Early Intervention With CO2 Ablative Fractional Laser to Improve Surgical Scars.

IF 2.2 3区医学 Q2 DERMATOLOGY

Dermatologic Surgery Pub Date : 2025-08-25 DOI:10.1097/DSS.0000000000004732

Jiaxing Liu, Ruishuang Sun, Yingxia Luo, Liujie Shi, Yunsong Zhang

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引用次数: 0

Abstract

Background: Scarless healing has always been the ultimate goal of surgery. Early intervention with a CO2 ablative fractional laser (CO2 AFL) has been found to improve the appearance of surgical scars.

Objective: To investigate the mechanism of early intervention with a CO2 AFL in promoting skin wound healing and the role of follicular stem cells.

Methods: The authors used seven-week-old male LGR5-Cre, Rosa-mtmg (LGR5-mtmg), krt14-gfp-creer2, and BALBc mice to establish a skin incision model and apply CO2 AFL treatment. The histological analysis and transcriptome sequencing were performed, and the results were verified using RT-PCR and western blotting. Transgenic fluorescent mice expressing Lgr5 and K14 were used to follow the trajectories of hair follicle stem cells and epidermal stem cells during wound healing.

Results: Histological analyses revealed that after CO2 AFL treatment, the number of incision marks in the incision tissue was lower. After treatment, the number of Lgr6 and Lgr5+ hair follicle stem cells and K14 epidermal stem cells increased, and the expression of Trps1 and its downstream Wnt pathway increased.

Conclusion: Early intervention with a CO2 AFL to promote skin incision healing in mice may be achieved by activating Trps1 to regulate the Wnt/β-catenin pathway and promote hair follicle stem-cell participation in skin wound healing.

查看原文本刊更多论文

CO2烧蚀分形激光早期干预改善手术瘢痕的机制探讨。

背景：无疤痕愈合一直是外科手术的终极目标。早期干预与CO2烧蚀分数激光（CO2 AFL）已被发现改善外观的手术疤痕。目的：探讨CO2 AFL早期干预促进皮肤创面愈合的机制及卵泡干细胞的作用。方法：选用7周龄雄性LGR5-Cre、Rosa-mtmg （LGR5-mtmg）、krt14-gfp-creer2、BALBc小鼠建立皮肤切口模型，进行CO2 AFL治疗。进行组织分析和转录组测序，并采用RT-PCR和western blotting验证结果。利用表达Lgr5和K14的转基因荧光小鼠追踪毛囊干细胞和表皮干细胞在伤口愈合过程中的轨迹。结果：组织学分析显示，CO2 AFL治疗后，切口组织中切口标记数量减少。治疗后，Lgr6、Lgr5+毛囊干细胞和K14表皮干细胞数量增加，Trps1及其下游Wnt通路表达增加。结论：CO2 AFL早期干预促进小鼠皮肤切口愈合可能通过激活Trps1调控Wnt/β-catenin通路，促进毛囊干细胞参与皮肤创面愈合实现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dermatologic Surgery 医学-皮肤病学

CiteScore

3.10

自引率

16.70%

发文量

547

期刊介绍： Exclusively devoted to dermatologic surgery, the Dermatologic Surgery journal publishes the most clinically comprehensive and up-to-date information in its field. This unique monthly journal provides today’s most expansive and in-depth coverage of cosmetic and reconstructive skin surgery and skin cancer through peer-reviewed original articles, extensive illustrations, case reports, ongoing features, literature reviews and correspondence. The journal provides information on the latest scientific information for all types of dermatologic surgery including: -Ambulatory phlebectomy- Blepharoplasty- Body contouring- Chemical peels- Cryosurgery- Curettage and desiccation- Dermabrasion- Excision and closure- Flap Surgery- Grafting- Hair restoration surgery- Injectable neuromodulators- Laser surgery- Liposuction- Microdermabrasion- Microlipoinjection- Micropigmentation- Mohs micrographic surgery- Nail surgery- Phlebology- Sclerotherapy- Skin cancer surgery- Skin resurfacing- Soft-tissue fillers. Dermatologists, dermatologic surgeons, plastic surgeons, oculoplastic surgeons and facial plastic surgeons consider this a must-read publication for anyone in the field.