Primary cilium disappearance in podocytes during vertebrate phylogeny revealed by array tomography.

IF 2.9 3区 生物学 Q3 CELL BIOLOGY
Cell and Tissue Research Pub Date : 2025-10-01 Epub Date: 2025-08-29 DOI:10.1007/s00441-025-04002-z
Takayuki Miyaki, Jingyuan Xu, Makoto Sugiura, Hisako Kaneda, Juan Alejandro Oliva Trejo, Yuto Kawasaki, Takuya Omotehara, Takako Negishi-Koga, Muneaki Ishijima, Junji Yamaguchi, Soichiro Kakuta, Koichiro Ichimura
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Abstract

Podocytes are a type of epithelial cells that form the kidney nephron and are essential for ultrafiltration in the glomerulus. The majority of nephron constituent cells have primary cilia, which play an important role in the maintenance of normal tubular architecture of nephron. However, whether podocytes have primary cilia was only partially understood. In general, immunohistochemistry with an anti-acetylated α-tubulin antibody is often used to visualize primary cilia. α-Tubulin is highly acetylated throughout podocytes, and this antibody is not suitable to determine the presence or absence of primary cilia in podocytes, which is one reason why the presence of primary cilia in podocytes has remained unclear. In the present study, we determined the presence or absence of primary cilia in mature podocytes of six vertebrate species using a recently reported array tomography workflow optimized for whole glomerulus analysis. The proportion of podocytes with primary cilia tended to decrease with evolution as follows: 89.6% in river lampreys (Agnatha), 42.1% in zebrafish (Osteichthyes), 43.3% in African clawed frogs (Amphibia), 17.3% in Reeves' turtles (Reptilia), 10.4% in common quails (Aves), and 0.0% in Wistar rats (Mammalia). Our previous study has reported that, in rats, primary cilia are present in podocytes during development and disappear in mature podocytes. In other words, primary cilia disappear from podocytes during both phylogeny and ontogeny in vertebrates. We discuss the triggers and significance of primary cilium disappearance from vertebrate podocytes.

阵列断层扫描揭示脊椎动物系统发育过程中足细胞初级纤毛的消失。
足细胞是形成肾元的一种上皮细胞,对肾小球的超滤至关重要。大多数肾元组成细胞都有初级纤毛,它在维持肾元正常的管状结构中起着重要的作用。然而,足细胞是否有初级纤毛只是部分了解。通常,免疫组织化学与抗乙酰化α-微管蛋白抗体常用于观察初级纤毛。α-微管蛋白在足细胞中高度乙酰化,该抗体不适合测定足细胞中初级纤毛的存在与否,这也是目前尚不清楚足细胞中初级纤毛是否存在的原因之一。在本研究中,我们使用最近报道的阵列断层扫描工作流程来确定六种脊椎动物成熟足细胞中初级纤毛的存在或缺失。具有初级纤毛的足细胞所占比例随进化呈下降趋势:七鳃鳗(Agnatha)占89.6%,斑马鱼(Osteichthyes)占42.1%,非洲爪蛙(amphi纲)占43.3%,里氏龟(爬行纲)占17.3%,鹌鹑(Aves)占10.4%,Wistar大鼠(哺乳纲)占0.0%。我们之前的研究报道,在大鼠中,初级纤毛在发育过程中存在于足细胞中,在成熟足细胞中消失。换句话说,在脊椎动物的系统发育和个体发育过程中,初级纤毛从足细胞中消失。我们讨论了脊椎动物足细胞初级纤毛消失的触发因素和意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell and Tissue Research
Cell and Tissue Research 生物-细胞生物学
CiteScore
7.00
自引率
2.80%
发文量
142
审稿时长
1 months
期刊介绍: The journal publishes regular articles and reviews in the areas of molecular, cell, and supracellular biology. In particular, the journal intends to provide a forum for publishing data that analyze the supracellular, integrative actions of gene products and their impact on the formation of tissue structure and function. Submission of papers with an emphasis on structure-function relationships as revealed by recombinant molecular technologies is especially encouraged. Areas of research with a long-standing tradition of publishing in Cell & Tissue Research include: - neurobiology - neuroendocrinology - endocrinology - reproductive biology - skeletal and immune systems - development - stem cells - muscle biology.
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