Cicer arietinum extract as antitumor and protective agent against Ehrlich Solid Carcinoma-bearing mice.

IF 3.4 2区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

BMC Complementary Medicine and Therapies Pub Date : 2025-09-02 DOI:10.1186/s12906-025-05061-z

Amany Ahmed Sayed, Mahmoud Salah Abdullah, Amr Ahmed WalyEldeen, Rasha Mohamed Samir Sayed, Refaat M Gabre, Sherif Abdelaziz Ibrahim, Hebatallah Hassan

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Abstract

Background: Cancer remains a significant global health challenge. Several plant-derived compounds have garnered the attention of cancer research for their anticancer effects. Chickpea (Cicer arietinum) is one of the top nutritious legumes with promising chemoprotective effects. We previously demonstrated that Cicer arietinum extract (CAE) has antitumor activity in vitro. Therefore, herein, we aimed to extend our findings and investigate CAE's preventive and therapeutic antitumor effects in vivo using a murine Ehrlich solid carcinoma (ESC) model.

Methods: Thirty-six female mice were divided into six groups: healthy controls, untreated ESC-bearing mice, CAE pre-treated groups (low, moderate, and high doses), and CAE post-treated group administered after tumor establishment. Tumor size, oxidative stress markers, antioxidant enzyme activities, and the expression of apoptotic (Casp3)- and pyroptotic (Gsdmd)-related genes were assessed. Finally, the immunohistochemical staining for the anti-apoptotic Survivin expression was assessed across different mice groups.

Results: Our data indicate that pre-treatment with moderate and high doses of CAE, as well as post-treatment, significantly inhibited tumor growth by more than 40% relative to untreated ESC-bearing controls. Histopathological analysis showed notable improvements in muscle tissue structure in CAE-treated samples. Mechanistically, CAE exerted its chemopreventive and therapeutic effects via the alleviation of oxidative stress by a significant enhancement of the antioxidant enzyme activities and increased Gpx4 mRNA expression, accompanied by a reduction of MDA and NO levels. Furthermore, CAE attenuated survivin expression, while dramatically boosting the expression of apoptotic Casp3 and pyroptotic Gsdmd markers, particularly in the post-treatment group.

Conclusion: These findings suggest that CAE is a promising anticancer agent with protective effects against ESC.

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枸杞子提取物对荷瘤小鼠的抗肿瘤和保护剂作用。

背景：癌症仍然是一个重大的全球健康挑战。几种植物衍生的化合物因其抗癌作用而引起了癌症研究的注意。鹰嘴豆（Cicer arietinum）是一种营养丰富的豆类植物，具有良好的化学保护作用。我们在体外实验中证实了枸杞提取物（CAE）具有抗肿瘤活性。因此，在此，我们旨在扩展我们的发现，并通过小鼠埃利希实体癌（ESC）模型研究CAE在体内的预防和治疗抗肿瘤作用。方法：将36只雌性小鼠分为6组：健康对照组、未处理的esc -荷瘤小鼠、CAE预处理组（低、中、高剂量）和肿瘤建立后给药CAE后处理组。评估肿瘤大小、氧化应激标志物、抗氧化酶活性以及凋亡（Casp3）和焦亡（Gsdmd）相关基因的表达。最后，通过免疫组化染色检测各组小鼠抗凋亡Survivin的表达。结果：我们的数据表明，与未治疗的ESC-bearing对照组相比，治疗前使用中剂量和高剂量CAE以及治疗后显著抑制肿瘤生长超过40%。组织病理学分析显示，cae处理样品的肌肉组织结构有显著改善。机制上，CAE通过显著增强抗氧化酶活性，增加Gpx4 mRNA表达，同时降低MDA和NO水平，从而减轻氧化应激，发挥化学预防和治疗作用。此外，CAE降低了survivin的表达，同时显著提高了凋亡的Casp3和焦亡的Gsdmd标志物的表达，尤其是在治疗后组。结论：CAE是一种很有前景的抗癌药物，对ESC具有保护作用。

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