Titanium Implants Functionalized with Zoledronic Acid Associated with Ruterpy Accelerate Peri-Implant Repair in Healthy and Osteoporotic Rats.

IF 3.9 3区医学 Q1 ENGINEERING, MULTIDISCIPLINARY

Biomimetics Pub Date : 2025-08-20 DOI:10.3390/biomimetics10080547

Laura Vidoto Paludetto, Isadora Breseghello, Sabrina Cruz Tfaile Frasnelli, Fábio Roberto de Souza Batista, Paulo Roberto Botacin, Cristina Antoniali, Paulo Noronha Lisboa-Filho, Roberta Okamoto

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Abstract

Osteoporosis compromises bone quality and impairs implant osseointegration. Since an adequate bone bed is essential for implant stability and success, this study evaluated the effects of implant surface functionalization with zoledronic acid (ZOL), alone or combined with ruterpy (TERPY), on peri-implant bone healing in healthy (SHAM) and osteoporotic (OVX) rats. ZOL has antiresorptive properties, while TERPY exhibits osteoinductive potential. The hypothesis was that ZOL + TERPY would act synergistically by inhibiting bone resorption and promoting new bone formation. Sixty-six female Wistar rats (3 months old) were divided into six groups (n = 11) according to systemic condition (SHAM or OVX) and implant type: conventional (CONV), ZOL, or ZOL + TERPY. Surgeries (sham or bilateral ovariectomy) were performed on day 0, and implants were placed in the tibial metaphysis on day 90. Fluorochromes were administered on days 104 (calcein) and 114 (alizarin), and euthanasia was performed on day 118. Samples were analyzed histologically via confocal microscopy and micro-computed tomography (Micro-CT). The ZOL + TERPY groups demonstrated significantly accelerated peri-implant bone repair, showing greater bone formation and organization; improved BV/TV, Tb.N, and I.S.; and reduced Tb.Sp and Po.Tot compared to CONV and ZOL-alone groups. In conclusion, ZOL + TERPY enhances and speeds bone healing, even under osteoporotic conditions.

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与Ruterpy相关的唑来膦酸功能化钛种植体加速健康和骨质疏松大鼠种植体周围修复。

骨质疏松损害骨质量，损害种植体骨整合。由于足够的骨床对种植体的稳定性和成功至关重要，本研究评估了唑来膦酸（ZOL）单独或联合ruterpy （TERPY）对健康（SHAM）和骨质疏松（OVX）大鼠种植体周围骨愈合的影响。ZOL具有抗骨吸收特性，而TERPY具有骨诱导潜能。假设ZOL + TERPY通过抑制骨吸收和促进新骨形成协同作用。雌性Wistar大鼠66只（3月龄），根据全身情况（SHAM或OVX）和种植体类型分为常规（CONV）、ZOL、ZOL + TERPY 6组（n = 11）。第0天进行手术（假或双侧卵巢切除术），第90天在胫骨干骺端放置植入物。第104天（钙黄素）和第114天（茜素）给予荧光染料，第118天进行安乐死。通过共聚焦显微镜和显微计算机断层扫描（Micro-CT）对样品进行组织学分析。ZOL + TERPY组明显加速种植体周围骨修复，显示出更大的骨形成和组织；改进的BV/TV， Tb美国和美国；减少结核病。Sp和Po。与CONV组和单独zol组比较。总之，即使在骨质疏松的情况下，ZOL + TERPY也能增强和加速骨愈合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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