Quantitation of Arsenic in biological matrices: Use of Sector Field Inductively Coupled Plasma-Mass Spectrometry for Improved Accuracy and Specificity.

IF 1.8 4区 化学 Q3 CHEMISTRY, ANALYTICAL
Chamindu Liyanapatirana, Frank X Weber, James M Harrington, Laura G Haines, Reshan A Fernando, Keith E Levine, Suramya Waidyanatha
{"title":"Quantitation of Arsenic in biological matrices: Use of Sector Field Inductively Coupled Plasma-Mass Spectrometry for Improved Accuracy and Specificity.","authors":"Chamindu Liyanapatirana, Frank X Weber, James M Harrington, Laura G Haines, Reshan A Fernando, Keith E Levine, Suramya Waidyanatha","doi":"10.1080/00032719.2025.2496934","DOIUrl":null,"url":null,"abstract":"<p><p>Arsenic (As) and As-containing compounds have a wide array of applications in medicine, industry, and agriculture and has been associated with adverse health outcomes. In support of studies investigating the toxicity and toxicokinetic behavior of arsenic compounds, we developed and validated a high-resolution sector field inductively coupled plasma mass spectrometry (SF-ICP-MS) method to quantitate total arsenic in Sprague-Dawley (SD) rat plasma and heart. Primary matrix standard curves prepared in male SD rat plasma were linear (r ≥ 0.999) over the range of 5 to 1250 ng As/mL plasma with accuracy determined as relative error (RE) was ≤ ±20% at the lower limit of quantitation (LLOQ) of 5 ng/mL and ≤ ±15% for all other concentrations. The method limit of detection (LOD) was 0.728 ng/mL plasma. Mean extraction recovery was 96.7%, with a relative standard deviation (RSD) of 4.0%. The method demonstrated acceptable inter- (≤ ±7.3% RE and ≤ 8.0% RSD) and intra-day (≤ ±14.9% RE and ≤ 6.8% RSD) accuracy and precision. Method selectivity was ≤ 20% of the response for the LLOQ. Method performance was also evaluated in SD rat heart by preparing QC samples in heart homogenate and quantifying using plasma calibration curve. The mean RE value was -3.8% and the RSD value was 3.4% demonstrating that the method is suitable for quantitation of arsenic in rat heart.</p>","PeriodicalId":7788,"journal":{"name":"Analytical Letters","volume":" ","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12383451/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Letters","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1080/00032719.2025.2496934","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Arsenic (As) and As-containing compounds have a wide array of applications in medicine, industry, and agriculture and has been associated with adverse health outcomes. In support of studies investigating the toxicity and toxicokinetic behavior of arsenic compounds, we developed and validated a high-resolution sector field inductively coupled plasma mass spectrometry (SF-ICP-MS) method to quantitate total arsenic in Sprague-Dawley (SD) rat plasma and heart. Primary matrix standard curves prepared in male SD rat plasma were linear (r ≥ 0.999) over the range of 5 to 1250 ng As/mL plasma with accuracy determined as relative error (RE) was ≤ ±20% at the lower limit of quantitation (LLOQ) of 5 ng/mL and ≤ ±15% for all other concentrations. The method limit of detection (LOD) was 0.728 ng/mL plasma. Mean extraction recovery was 96.7%, with a relative standard deviation (RSD) of 4.0%. The method demonstrated acceptable inter- (≤ ±7.3% RE and ≤ 8.0% RSD) and intra-day (≤ ±14.9% RE and ≤ 6.8% RSD) accuracy and precision. Method selectivity was ≤ 20% of the response for the LLOQ. Method performance was also evaluated in SD rat heart by preparing QC samples in heart homogenate and quantifying using plasma calibration curve. The mean RE value was -3.8% and the RSD value was 3.4% demonstrating that the method is suitable for quantitation of arsenic in rat heart.

生物基质中砷的定量:使用扇形场电感耦合等离子体质谱法提高准确性和特异性。
砷(As)和含砷化合物在医学、工业和农业中有着广泛的应用,并与不良的健康后果有关。为了支持研究砷化合物的毒性和毒性动力学行为,我们开发并验证了一种高分辨率扇形场电感耦合等离子体质谱(SF-ICP-MS)方法,用于定量SD大鼠血浆和心脏中的总砷。在雄性SD大鼠血浆中制备的初级基质标准曲线在5 ~ 1250 ng As/mL血浆范围内呈线性(r≥0.999),在定量下限(LLOQ)为5 ng/mL时,相对误差(RE)≤±20%,其他浓度均≤±15%。方法检出限(LOD)为0.728 ng/mL。平均提取回收率为96.7%,相对标准偏差(RSD)为4.0%。该方法具有可接受的间(≤±7.3% RE,≤8.0% RSD)和日内(≤±14.9% RE,≤6.8% RSD)准确度和精密度。方法选择性≤20%的LLOQ响应。用心脏匀浆制备QC样品,用血浆校准曲线定量,评价方法在SD大鼠心脏中的性能。平均RE值为-3.8%,RSD值为3.4%,表明该方法适用于大鼠心脏中砷的定量测定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Analytical Letters
Analytical Letters 化学-分析化学
CiteScore
4.10
自引率
5.00%
发文量
218
审稿时长
2.1 months
期刊介绍: Analytical Letters is an international medium for the rapid publication of original research papers, accelerated articles, or mini-reviews on important developments in all areas of analytical chemistry, including electrochemistry, mass spectrometry, separations, and spectroscopy. Papers are welcomed that consider fundamental developments, new or improved instrumentation and sensors, and applications of analytical chemistry in all areas that include biological and clinical science, engineering and instrumentation science, environmental chemistry and analysis, geochemistry, materials science, nanotechnology, and physics.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信