LC-QTOF-MS/MS for tentative identification of antioxidant peptides in defatted Spirulina platensis cakes obtained by supercritical CO2 extraction.

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Lucía Cuesta-Ramos, Nerea Sánchez-Moreno, Manuel Salgado-Ramos, Juan Manuel Castagnini, Francisco J Martí-Quijal, Emilia Ferrer, Francisco J Barba, Noelia Pallarés
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Abstract

This work tackles a supercritical fluid extraction (SFE) of microalga spirulina (Arthrospira platensis) to recover potential products from a nutritional and bioactive point of view. The execution of a response-surface model (RSO) through Box-Behnken design yielded 86% defatting, which significantly contrasts traditional Soxhlet extraction (9%). Contrarily to most of the reported works in the literature that focused on the analysis of the recovered oil, this study points out the added value of the remaining cakes after processing (spirulina residues). Namely, protein content determined by the DUMAS method concluded that a noted increase in the SFE cake was produced with respect to the raw material (from 69.2 to 74.4 g/100 g). In this regard, a tentative identification of antioxidant peptides through LC-QTOF-MS/MS was carried out. More deeply, 14 peptide sequences containing specific amino acids with potential antioxidant capacity were found in the SFE remaining cakes, of which the peptide sequences "SIVNADAEARYLSPGELDRIK" and "RYLSPGELDRIKSFVT" were only detected in these cakes and not in the Soxhlet solids. In addition, 4 short amino acid groups associated with potential antioxidant capacity were also identified in both cases ("EL", "RY", "RYL", and "YL"). ICP-MS served to determine the mineral profile in these materials, with most of them remaining in the cakes after SFE and Soxhlet extractions. With respect to oils, they were rich in Mg, P, K, and Fe minerals. The SFE oil extract had a much higher antioxidant capacity, phenolic content, chlorophyll A, and carotenoid content than the Soxhlet extract. Overall, this study presents an advanced extraction technique that efficiently recovers bioactive compounds and nutrients with potential food applications.

超临界CO2萃取脱脂螺旋藻饼中抗氧化肽的LC-QTOF-MS/MS初步鉴定。
从营养和生物活性的角度对螺旋藻(Arthrospira platensis)进行超临界流体萃取(SFE),以回收潜在的产品。通过Box-Behnken设计执行响应面模型(RSO),脱脂率为86%,与传统索氏提取(9%)相比显著提高。与大多数文献报道的集中分析回收油的工作相反,本研究指出了加工后剩余蛋糕(螺旋藻残留物)的附加值。也就是说,通过DUMAS法测定的蛋白质含量表明,相对于原料,SFE饼的产量显著增加(从69.2 g/100 g增加到74.4 g/100 g)。为此,采用LC-QTOF-MS/MS对抗氧化肽进行了初步鉴定。更深入地说,在SFE剩余饼中发现了14个含有潜在抗氧化能力的特定氨基酸的肽序列,其中“SIVNADAEARYLSPGELDRIK”和“RYLSPGELDRIKSFVT”肽序列仅在这些饼中检测到,而在索氏固体中未检测到。此外,在两种情况下还鉴定出4个与潜在抗氧化能力相关的短氨基酸基团(“EL”,“RY”,“RYL”和“YL”)。ICP-MS用于测定这些材料中的矿物分布,经SFE和索氏萃取后,大部分矿物仍留在饼中。油类中含有丰富的镁、磷、钾、铁等矿物。SFE油提取物的抗氧化能力、酚类含量、叶绿素a和类胡萝卜素含量均高于索氏提取物。总之,本研究提出了一种先进的提取技术,可以有效地回收生物活性化合物和营养物质,具有潜在的食品应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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