Photonic resonator absorption microscopy: why consider metallic and magneto-plasmonic nano-assemblies over bare nanoparticles for digital biosensing?

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Skye Shepherd, Weinan Liu, Seemesh Bhaskar, Brian T Cunningham
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引用次数: 0

Abstract

The unique optical interaction of species such as nanomaterials, proteins, viruses, antibodies, microRNA, and exosomes with the one-dimensional grating-based photonic crystals (PCs) has been leveraged in their detection using photonic crystal absorption microscopy (PRAM). While the principle and fundamental mechanism of such interfacial interactions are well delineated using wavelength and intensity modulations associated with the guided-mode resonance (GMR) of the PC, the effect of nano-assemblies in place of nanoparticles (NPs) has not been reported previously. In this work, the fundamental limitations observed with pristine NPs are overcome through the use of tunable AuNP assemblies synthesized via adiabatic cooling technology, where tunable nano-assemblies are obtained by subjecting the respective NPs to - 196 °C. Moreover, the higher contrast rendered by magneto-plasmonic, Fe3O4-Au hybrid nano-assemblies vis-à-vis metallic AuNP assemblies is corroborated with COMSOL Multiphysics simulations using electric and magnetic field hotspots. The high-contrast digital resolution enabled by magneto-plasmonic hybrid nano-assemblies, on account of synergistic coupling between the GMR of the underlying PC, delocalized Bragg, and localized Mie plasmons of dielectric-metal nano-assemblies, demonstrated excellent performance for microRNA-375-3p detection, opening a new window to explore hybrids of tunable "permittivity + permeability" as active probes in the design and development of microscopy-based biosensing modalities.

光子谐振器吸收显微镜:为什么考虑金属和磁等离子体纳米组件而不是裸纳米粒子用于数字生物传感?
纳米材料、蛋白质、病毒、抗体、microRNA和外泌体等物种与一维光栅光子晶体(PCs)的独特光学相互作用已被利用在光子晶体吸收显微镜(PRAM)的检测中。虽然这种界面相互作用的原理和基本机制是通过与PC的导模共振(GMR)相关的波长和强度调制来很好地描述的,但纳米组件代替纳米颗粒(NPs)的效果以前没有报道过。在这项工作中,通过使用通过绝热冷却技术合成的可调AuNP组件来克服原始NPs观察到的基本限制,其中通过将各自的NPs置于- 196°C获得可调纳米组件。此外,通过COMSOL多物理场模拟,利用电场和磁场热点,证实了磁等离子体Fe3O4-Au混合纳米组件与-à-vis金属AuNP组件的更高对比度。由于底层PC的GMR、离域Bragg和介电-金属纳米组件的定域Mie等离子体之间的协同耦合,磁等离子体混合纳米组件实现了高对比度数字分辨率,显示了对microRNA-375-3p检测的优异性能,为探索可调“介电常数+磁导率”的混合体作为基于显微镜的生物传感模式设计和开发的主动探针打开了新的窗口。
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来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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