Illuminating New Frontiers: Exploring the Photosensitizing Potential of Passiflora Species in Combating Methicillin-Resistant Staphylococcus aureus (MRSA) and Their Infection in Senescent Mice.

IF 2.6 3区 生物学 Q3 MATERIALS SCIENCE, BIOMATERIALS
Caroline Vieira Gonçalves, Maria Poliana Leite Galantini, Igor Pereira Ribeiro Muniz, Paulo Henrique Bispo Lima, Israel Souza Ribeiro, Maria Eduarda Santos de Oliveira, Caio Oliveira Lopes de Magalhães, Maria Elisa Santos Flores, Samara Lopes de Oliveira, Catarina Silva Guimarães, Paulinne Moreira Lima, Luísa Carregosa Santos, Daiana Silva Lopes, Juliano Geraldo Amaral, Robson Amaro Augusto da Silva
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Abstract

Antimicrobial Photodynamic Therapy (aPDT) has become a potential alternative for treating multidrug-resistant bacterial skin infections, such as those caused by methicillin-resistant Staphylococcus aureus (MRSA), which are at high risk in aging individuals. One of the main components of aPDT is an agent known as a photosensitizer (PS). Some plants with high flavonoid content are reported as PS. In the genus Passiflora, flavonoids are predominant, but their photosensitizing activity has yet to be described. This study investigates the photosensitizing potential of extracts from Passiflora edulis, Passiflora alata, and Passiflora cincinnata. The butanolic fraction of P. cincinnata undergoes in vivo evaluation against intradermal MRSA infection in a senescent murine model (C57BL/6). In vitro assays determine the photoactivatable concentrations and their cytotoxicity. In vivo, MRSA-infected mice are divided into control, P. cincinnata-treated, and aPDT-treated groups. Subsequent assessments include cytokine levels, bacterial load, and cellular infiltrate in the ear. The P. cincinnata-treated group exhibits improved bacterial control, reduced leukocyte infiltration, and less weight loss. The aPDT group demonstrates a unique cytokine correlation profile, featuring more negative correlations among pro-inflammatory cytokines and interleukin-10. P. cincinnata emerges as an effective photosensitizer for aPDT in a senescent model and highlights the potential of underexplored plant-derived photosensitizers.

照亮新领域:探索西番莲属植物对抗耐甲氧西林金黄色葡萄球菌(MRSA)及其在衰老小鼠中的感染的光敏潜力。
抗菌素光动力疗法(aPDT)已成为治疗多重耐药细菌性皮肤感染的潜在替代方法,例如由耐甲氧西林金黄色葡萄球菌(MRSA)引起的皮肤感染,这些感染在老年人中具有高风险。aPDT的主要成分之一是一种称为光敏剂(PS)的药剂。在西番莲属植物中,黄酮类化合物占主导地位,但其光敏活性尚不清楚。本研究考察了西番莲、西番莲和辛辛那提西番莲提取物的光敏潜力。在衰老小鼠模型(C57BL/6)中,对蝎尾藤丁醇组分抗皮内MRSA感染进行了体内评估。体外试验确定光激活浓度及其细胞毒性。在体内,mrsa感染的小鼠被分为对照组、辛辛那提假单胞菌组和apdt组。随后的评估包括细胞因子水平、细菌负荷和耳内细胞浸润。辛辛那提假单胞菌处理组表现出更好的细菌控制,白细胞浸润减少,体重减轻。aPDT组表现出独特的细胞因子相关特征,促炎细胞因子与白细胞介素-10之间存在更多的负相关。在衰老模型中,P. cincinnati作为aPDT的有效光敏剂出现,并突出了未开发的植物源光敏剂的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Advanced biology
Advanced biology Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
6.60
自引率
0.00%
发文量
130
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