Rapid in vitro and computer-aided method for assessing synergistic interactions between NSAIDs and analgesics.

IF 4.3 3区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS
Fernando Silva, Gustavo Costa, Francisco Veiga, Vera Moura, Francisca Dias, Fátima Cerqueira, Rui Medeiros, Ana Cláudia Paiva-Santos
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引用次数: 0

Abstract

Pain is a complex phenomenon that plays a significant role in various diseases, influencing both the physical and psychological well-being of individuals. In clinical practice, combining nonsteroidal anti-inflammatory drugs (NSAIDs) with analgesics, such as paracetamol or metamizole, has become a widely adopted strategy to manage pain. Although the synergistic effects of combining NSAIDs with analgesics are well recognized in clinical practice, this approach is primarily based on empirical clinical experience. Our work aims to present a rapid method for evaluating the anti-inflammatory effects of drug combinations through in vitro assays combined with computer-aided data processing and analysis. We conducted two simple and rapid in vitro assays, the Griess and DPPH assays, to evaluate the effects of NSAID-analgesic combinations and demonstrate their synergistic interactions, using the free web application SynergyFinder Plus. This computer-aided analysis enabled a quantitative assessment of drug interactions, enhancing the interpretation of the experimental data. Furthermore, to better understand the results obtained from previous experiments, we analysed the anti-inflammatory effects of ketoprofen and dexketoprofen in combination with metamizole and paracetamol through quantitative real-time PCR (qRT-PCR). Our findings reveal synergistic interactions between NSAIDs and analgesics in terms of their anti-inflammatory and antioxidant activities. This work could be the first step for the study of the mechanisms behind the synergistic interactions between NSAIDs and analgesics for the treatment of pain, mainly when inflammatory processes are involved. Consequently, this study aims to contribute to the exploration of non-opioid drug combinations, addressing the urgent need for alternative analgesic strategies that minimize opioid use.

快速体外和计算机辅助方法评估非甾体抗炎药和镇痛药之间的协同相互作用。
疼痛是一种复杂的现象,在各种疾病中起着重要作用,影响着个体的生理和心理健康。在临床实践中,非甾体抗炎药(NSAIDs)与镇痛药(如扑热息痛或metamizole)联合使用已成为一种广泛采用的治疗疼痛的策略。尽管非甾体抗炎药与镇痛药联合使用的协同作用在临床实践中得到了广泛认可,但这种方法主要基于临床经验。我们的工作旨在通过体外试验结合计算机辅助数据处理和分析,提出一种快速评估药物联合抗炎作用的方法。我们使用免费的网络应用程序SynergyFinder Plus进行了两种简单快速的体外试验,Griess和DPPH试验,以评估nsaid -镇痛药联合使用的效果,并证明它们之间的协同作用。这种计算机辅助分析能够对药物相互作用进行定量评估,增强对实验数据的解释。此外,为了更好地理解之前的实验结果,我们通过实时荧光定量PCR (qRT-PCR)分析了酮洛芬和右酮洛芬与metamizole和paracetamol联合使用的抗炎作用。我们的发现揭示了非甾体抗炎药和镇痛药在抗炎和抗氧化活性方面的协同相互作用。这项工作可能是研究非甾体抗炎药和镇痛药治疗疼痛的协同相互作用机制的第一步,主要是在炎症过程中。因此,本研究旨在促进非阿片类药物组合的探索,解决替代镇痛策略的迫切需要,最大限度地减少阿片类药物的使用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Methods
Methods 生物-生化研究方法
CiteScore
9.80
自引率
2.10%
发文量
222
审稿时长
11.3 weeks
期刊介绍: Methods focuses on rapidly developing techniques in the experimental biological and medical sciences. Each topical issue, organized by a guest editor who is an expert in the area covered, consists solely of invited quality articles by specialist authors, many of them reviews. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting.
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