Encapsulating Pt NCs with an AIE effect into a metal-organic framework as a novel fluorescence nanoprobe for discriminatively detecting bisphosphonate drugs.

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Juan Du, Jinwen Chen, Huixiao Tong, Shenghua Liao
{"title":"Encapsulating Pt NCs with an AIE effect into a metal-organic framework as a novel fluorescence nanoprobe for discriminatively detecting bisphosphonate drugs.","authors":"Juan Du, Jinwen Chen, Huixiao Tong, Shenghua Liao","doi":"10.1007/s00216-025-06075-9","DOIUrl":null,"url":null,"abstract":"<p><p>Fluorescent metal organic frameworks (FMOFs) constructed by encapsulating aggregation-induced emission luminogens (AIEgens) into frameworks have garnered increasing attention due to their ability to overcome the aggregation-caused quenching (ACQ) effect of traditional fluorescent materials such as organic dyes, quantum dots, and up-conversion nanoparticles. However, conventional AIEgens usually contain aromatic groups that may pose potential health risks. Additionally, the quality of bisphosphonates (BPs), first-line treatment drugs in skeletal-related diseases, is critical to assure their effectiveness and safety. In this work, a new nanocomposite, Pt NCs@ZIF-8, was designed by encapsulating platinum nanoclusters (Pt NCs) with aggregation-induced emission (AIE) properties into zeolitic imidazolate framework-8 (ZIF-8) through self-assembly between Zn<sup>2+</sup> and Pt NCs. Owing to the spatial confinement provided by ZIF-8 and the AIE effect of Pt NCs, the resulting Pt NCs@ZIF-8 nanocomposite presents excellent photostability and anti-photobleaching. Furthermore, the framework of Pt NCs@ZIF-8 collapses in the presence of BP drugs due to the strong coordination interaction between the P = O groups in BPs and the Zn<sup>2+</sup> in ZIF-8, leading to the release of Pt NCs. This release of Pt NCs causes effective fluorescence quenching of the Pt NCs@ZIF-8 nanocomposite, resulting from the increasing intramolecular motion of surface ligands, glutathione (GSH), on Pt NCs. Therefore, a new Pt NCs@ZIF-8-based nanoprobe has been rationally designed for BP detection. More importantly, our designed Pt NCs@ZIF-8-based nanoprobe also presents excellent discrimination capability toward four structurally similar BPs-Etidronate, Clodronate, Alendronate, and Risedronate, as confirmed by statistical techniques such as principal component analysis (PCA) and hierarchical clustering analysis (HCA). Finally, the proposed Pt NCs@ZIF-8 nanoprobe was successfully applied to quantify the labeled amount percentage of commercially available Sodium Risedronate and Sodium Alendronate tablets, achieving satisfactory limits of detection (LOD) spanning from 1.2 to 8.8 µM and excellent recovery ranging from 97.50% to 102.6%. This work not only provides a novel and convenient strategy for detecting and distinguishing BPs but also demonstrates the potential application of metal-organic frameworks encapsulating metal nanoclusters with AIE properties in pharmaceutical quality control.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8000,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical and Bioanalytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s00216-025-06075-9","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

Fluorescent metal organic frameworks (FMOFs) constructed by encapsulating aggregation-induced emission luminogens (AIEgens) into frameworks have garnered increasing attention due to their ability to overcome the aggregation-caused quenching (ACQ) effect of traditional fluorescent materials such as organic dyes, quantum dots, and up-conversion nanoparticles. However, conventional AIEgens usually contain aromatic groups that may pose potential health risks. Additionally, the quality of bisphosphonates (BPs), first-line treatment drugs in skeletal-related diseases, is critical to assure their effectiveness and safety. In this work, a new nanocomposite, Pt NCs@ZIF-8, was designed by encapsulating platinum nanoclusters (Pt NCs) with aggregation-induced emission (AIE) properties into zeolitic imidazolate framework-8 (ZIF-8) through self-assembly between Zn2+ and Pt NCs. Owing to the spatial confinement provided by ZIF-8 and the AIE effect of Pt NCs, the resulting Pt NCs@ZIF-8 nanocomposite presents excellent photostability and anti-photobleaching. Furthermore, the framework of Pt NCs@ZIF-8 collapses in the presence of BP drugs due to the strong coordination interaction between the P = O groups in BPs and the Zn2+ in ZIF-8, leading to the release of Pt NCs. This release of Pt NCs causes effective fluorescence quenching of the Pt NCs@ZIF-8 nanocomposite, resulting from the increasing intramolecular motion of surface ligands, glutathione (GSH), on Pt NCs. Therefore, a new Pt NCs@ZIF-8-based nanoprobe has been rationally designed for BP detection. More importantly, our designed Pt NCs@ZIF-8-based nanoprobe also presents excellent discrimination capability toward four structurally similar BPs-Etidronate, Clodronate, Alendronate, and Risedronate, as confirmed by statistical techniques such as principal component analysis (PCA) and hierarchical clustering analysis (HCA). Finally, the proposed Pt NCs@ZIF-8 nanoprobe was successfully applied to quantify the labeled amount percentage of commercially available Sodium Risedronate and Sodium Alendronate tablets, achieving satisfactory limits of detection (LOD) spanning from 1.2 to 8.8 µM and excellent recovery ranging from 97.50% to 102.6%. This work not only provides a novel and convenient strategy for detecting and distinguishing BPs but also demonstrates the potential application of metal-organic frameworks encapsulating metal nanoclusters with AIE properties in pharmaceutical quality control.

将具有AIE效应的Pt - NCs包封在金属-有机框架中作为一种新型荧光纳米探针,用于鉴别检测双膦酸盐类药物。
荧光金属有机框架(FMOFs)通过将聚集致发射发光原(AIEgens)封装在框架中构建而成,由于其能够克服传统荧光材料(如有机染料、量子点和上转换纳米颗粒)的聚集致猝灭(ACQ)效应,受到越来越多的关注。然而,传统的空气源通常含有可能构成潜在健康风险的芳香族。此外,双膦酸盐(BPs)作为骨骼相关疾病的一线治疗药物,其质量对确保其有效性和安全性至关重要。在这项工作中,通过在Zn2+和Pt NCs之间的自组装,将具有聚集诱导发射(AIE)性质的铂纳米团簇(Pt NCs)封装到沸石咪唑盐框架-8 (ZIF-8)中,设计了一种新的纳米复合材料Pt NCs@ZIF-8。由于ZIF-8提供的空间约束和Pt NCs的AIE效应,得到的Pt NCs@ZIF-8纳米复合材料具有优异的光稳定性和抗光漂白性能。此外,由于BP中的P = O基团与ZIF-8中的Zn2+之间的强配位相互作用,Pt NCs@ZIF-8的框架在BP药物存在下崩溃,导致Pt nc的释放。Pt NCs的释放导致Pt NCs@ZIF-8纳米复合材料的有效荧光猝灭,这是由于表面配体谷胱甘肽(GSH)在Pt NCs上的分子内运动增加。因此,合理设计了一种新型Pt NCs@ZIF-8-based纳米探针用于BP检测。更重要的是,我们设计的Pt NCs@ZIF-8-based纳米探针也对四种结构相似的bp - etidronate, Clodronate, Alendronate和Risedronate具有出色的识别能力,这得到了主成分分析(PCA)和层次聚类分析(HCA)等统计技术的证实。最后,Pt NCs@ZIF-8纳米探针成功地用于定量市售利塞膦酸钠和阿仑膦酸钠片的标记量百分比,获得了令人满意的检出限(LOD),范围为1.2 ~ 8.8µM,回收率为97.50% ~ 102.6%。这项工作不仅为bp的检测和区分提供了一种新颖而便捷的策略,而且还展示了具有AIE特性的金属-有机框架封装金属纳米团簇在药物质量控制中的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信