Biomodification of coronal bovine dentin with chitosan solutions associated with modified nano-hydroxyapatite and Biosilicate®.

IF 6.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Dental Materials Pub Date : 2025-08-26 DOI:10.1016/j.dental.2025.08.006

Isabela Barbosa Quero, Pâmella Coelho Dias, Francisco Wanderley Garcia de Paula E Silva, Nilza Letícia Magalhães, Juliana Jendiroba Faraoni, Regina Guenka Palma-Dibb

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引用次数: 0

Abstract

Objectives: This study aimed to test experimental chitosan-based solutions, with modified nano-hydroxyapatite (n-HA) and Biosilicate as mineral sources with and without L-Aspartic acid (L-Asp) as the polymer-induced liquid-precursor (PILP), on mineral deposition and activity and expression of matrix metalloproteinase (MMP) on demineralized coronal bovine dentin and collagen fiber reinforcement.

Methods: Six chitosan-based experimental gel solutions were used as treatment: Sol1-2 % chitosan; Sol2-2 % chitosan+ 5.5 % n-HA; Sol3-2 % chitosan+ 0.02 % L-Asp+ 5.5 % n-HA; Sol4-2 % chitosan+ 1 % Biosilicate; Sol5-2 % chitosan+ 0.02 % L-Asp+ 1 % Biosilicate; Sol6-2 % chitosan+ 0.02 % L-Asp. Demineralized bovine dentin specimens and collagen fiber were treated for 5 min and immersed in artificial saliva for 14 days. Dentin fragments were analyzed to determine their chemical composition with Attenuated total reflectance - Fourier transform infrared spectroscopy (ART-FTIR) and to evaluate the activity and expression of two key gelatinases (MMP-2 and MMP-9) with zymography and immunofluorescence. Dentin slices were used to assess dentin density by transmitted light microscopy. Collagen fiber was tested through microtensile test. Data analysis was performed by ANOVA and Kruskal-Wallis.

Results: Samples treated with Sol1 and Sol3 showed more carbonate after demineralization; and Sol2 and Sol3 presented the highest values of collagen cross-link. The gelatinolytic activity of Sol2 and Sol3 showed statistically similar results to sound dentin (p > 0.05). Sol1, 2, and 4 resulted in a higher mineral density. Sol2, 3, and 6 showed the highest values for tensile strength.

Significance: Therefore, the treatment of demineralized dentin with modified nano-hydroxyapatite resulted in the reinforcement of collagen fiber, mineral deposition in dentin architecture, and the inhibitor of metalloproteinases.

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壳聚糖与改性纳米羟基磷灰石和生物硅酸盐结合的冠状牛牙本质生物改性。

目的：以改性纳米羟基磷灰石（n-HA）和生物硅酸盐为矿物来源，以l -天冬氨酸（L-Asp）为聚合物诱导液体前体（PILP），研究壳聚糖为基础的实验溶液在脱矿牛冠状牙本质和胶原纤维增强上的沉积、基质金属蛋白酶（MMP）的活性和表达。方法：采用6种壳聚糖基实验凝胶溶液处理：Sol1-2 %壳聚糖；Sol2-2 %壳聚糖+ 5.5% % n-HA；Sol3-2 %壳聚糖+ 0.02 % L-Asp+ 5.5 % n-HA；溶胶4-2 %壳聚糖+ 1 %生物硅酸盐；Sol5-2 %壳聚糖+ 0.02 % L-Asp+ 1 %生物硅酸盐；Sol6-2 %壳聚糖+ 0.02 % L-Asp。脱矿后的牛牙本质标本和胶原纤维处理5 min，浸泡在人工唾液中14 d。用衰减全反射-傅里叶变换红外光谱（ART-FTIR）分析牙本质片段的化学成分，用酶谱法和免疫荧光法评价两种关键明胶酶（MMP-2和MMP-9）的活性和表达。牙本质切片采用透射光镜检查牙本质密度。通过微拉伸试验检测胶原纤维。数据分析采用方差分析和Kruskal-Wallis分析。结果：经Sol1和Sol3处理的样品脱矿后碳酸盐含量增加；其中，Sol2和Sol3胶原交联率最高。Sol2和Sol3的溶胶活性与正常牙本质相似（p > 0.05）。Sol1、2和4导致较高的矿物质密度。Sol2、3、6的抗拉强度最高。意义：因此，用改性纳米羟基磷灰石处理脱矿牙本质可以增强胶原纤维，在牙本质结构中沉积矿物质，抑制金属蛋白酶。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dental Materials 工程技术-材料科学：生物材料

CiteScore

9.80

自引率

10.00%

发文量

290

审稿时长

67 days

期刊介绍： Dental Materials publishes original research, review articles, and short communications. Academy of Dental Materials members click here to register for free access to Dental Materials online. The principal aim of Dental Materials is to promote rapid communication of scientific information between academia, industry, and the dental practitioner. Original Manuscripts on clinical and laboratory research of basic and applied character which focus on the properties or performance of dental materials or the reaction of host tissues to materials are given priority publication. Other acceptable topics include application technology in clinical dentistry and dental laboratory technology. Comprehensive reviews and editorial commentaries on pertinent subjects will be considered.