GsSnRK1.1 Kinase Positively Regulates the Glycine soja Nitrate Transporter GsNRT2.4a in Response to Nitrogen Starvation.

IF 6.3 1区 生物学 Q1 PLANT SCIENCES
Minglong Li, Hongguang You, Wenya Jiang, Shixi Lu, Yuechuan Hou, Jialei Xiao, Weizhong Zeng, Pengfei Xu, Xiaodong Ding, Xiuju Wu, Shuzhen Zhang, Qiang Li
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Abstract

Wild soybean (Glycine soja) is a leguminous species known for its ability to thrive in challenging and barren environments. It has been reported that the nitrate transporters (NRTs) play critical roles for plants to survive in the nutrient-poor soils. However, the molecular mechanisms of GsNRTs in governing nitrogen (N) uptake remain largely elusive. In the present study, we identified a NRT2.4-like protein (GsNRT2.4a) as an interactor of GsSnRK1.1 kinase. Our biophysical and physiological analyses indicate that GsNRT2.4a functions as an active NRT, and GsSnRK1.1 kinase phosphorylates the Ser518 residue at the carboxyl region of GsNRT2.4a. Under N starvation conditions, the double mutant nrt2.1/nrt2.2 (2nrtm) and the quadruple mutant nrt2.1/nrt2.2/kin10/kin11 (2kinm/2nrtm) exhibited compromised growth of Arabidopsis. However, introduction of GsNRT2.4a or GsSnRK1.1/GsNRT2.4a genes into the mutants rescued their defective growth to different extent. Furthermore, we determined that GsSnRK1.1 plays a pivotal role in modulating GsNRT2.4a activity in planta by phosphorylating GsNRT2.4a at the Ser518 site, thereby collaboratively modulating plant growth under N starvation. Our findings suggest that GsNRT2.4a is essential for optimising nitrate uptake in plants, and it also elucidates a novel regulatory mechanism of GsSnRK1.1-GsNRT2.4a module for potential enhancement of nitrogen use efficiency (NUE) in plants.

GsSnRK1.1激酶正调控甘氨酸大豆硝酸盐转运体GsNRT2.4a对氮饥饿的响应
野生大豆(甘氨酸大豆)是一种豆科植物,以其在具有挑战性和贫瘠的环境中茁壮成长的能力而闻名。硝酸盐转运体(nrt)对植物在养分贫乏的土壤中生存起着至关重要的作用。然而,GsNRTs调控氮(N)摄取的分子机制在很大程度上仍然是未知的。在本研究中,我们发现了一个nrt2.4样蛋白(GsNRT2.4a)作为GsSnRK1.1激酶的相互作用因子。我们的生物物理和生理分析表明,GsNRT2.4a是一个活性的NRT, GsSnRK1.1激酶磷酸化GsNRT2.4a羧基区的Ser518残基。在缺氮条件下,双突变体nrt2.1/nrt2.2 (2nrtm)和四突变体nrt2.1/nrt2.2/kin10/kin11 (2kinm/2nrtm)的拟南芥生长受到抑制。然而,将GsNRT2.4a或gsnrk1.1 /GsNRT2.4a基因导入突变体后,不同程度地挽救了突变体的生长缺陷。此外,我们确定GsSnRK1.1通过在Ser518位点磷酸化GsNRT2.4a,在植物中调节GsNRT2.4a活性中起关键作用,从而协同调节N饥饿下的植物生长。研究结果表明,gsnrk1.1 -GsNRT2.4a对优化植物对硝酸盐的吸收至关重要,并阐明了gsnrk1.1 -GsNRT2.4a模块在提高植物氮素利用效率(NUE)方面的新调控机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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