Dual Isotope Analysis Reveals Phylogenetic Patterns and Novel Insights Into Methoxy Group Synthesis of Structural Biomolecules in Leaf and Woody Plant Tissues.

Abstract

Stable carbon and hydrogen isotopes of wood methoxy groups (δ²H_meth, δ¹³C_meth), mainly sourced by structural biomolecules like lignin and pectin, provide important insights into climatic, hydrological and physiological conditions. This study systematically investigated species-specific δ²H_meth and δ¹³C_meth variations in leaves and woody twigs of 65 different tree species grown in a common garden. Significant phylogenetic patterns were observed in δ²H_meth and δ¹³C_meth of both tissues, with stronger signals in leaves and the most pronounced differences between angiosperms and gymnosperms. δ¹³C_meth variations are likely explained by anatomical and physiological differences between seed types, while δ²H_meth variations were attributed to temporal differences in water uptake or isotope fractionation processes. Notably, δ¹³C_meth values were more negative in leaves than in twigs, while δ²H_meth values showed no tissue-specific difference. This suggests that serine, a methoxy precursor, is differently synthesised in autotrophic than in heterotrophic tissues. Hydrogen isotope fractionation between xylem water and twig methoxy groups averaged at -197 mUr, with mean isotope fractionation of gymnosperms -209 mUr being significantly different to that of angiosperms -184 mUr. Weak relationships between δ²H_meth and δ²H values of carbohydrates indicated that distinct signals are preserved within the two compounds. This study highlights the importance of phylogenetic considerations when using methoxy group isotopes as proxies and provides new insights into methoxy group biosynthesis.