Endophytic Pseudomonas oryzihabitans CB24 Boosts Photosynthesis Through Monogalactosyldiacylglycerol Driven Lipid Reprogramming.

IF 6.3 1区 生物学 Q1 PLANT SCIENCES
Nikky Deepa, Shivam Chauhan, Prabodh K Trivedi, Akanksha Singh
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Abstract

Pelargonium graveolens, valued for its essential oil, is significantly influenced by its endosymbiotic associations impacting its physiology and phytochemistry, though the exact mechanisms driving this modulation remain largely unexplored. This study unveils that inoculating Pseudomonas oryzihabitans CB24 into P. graveolens significantly alters plant's lipid dynamics, leading to increased accumulation of chloroplast glycerolipids like monogalactosyldiacylglycerol (MGDG) and sulfolipids, sulfoquinovosyldiacylglycerol (SQDG). This is achieved by enhancing precursors like UDP-6-sulfoquinovose and acetyl CoA, alongside upregulating genes such as plc, MGD1, and SQD2 crucial for glycerolipid metabolism. Glycerolipids being essential for thylakoidal membrane stability resulted in significant upregulation of genes related to antennae protein, light-harvesting complexes namely, LHCA2, LHCA1, PsaA, PetE, PetF, PsbO. P. oryzihabitans also boosted RuBisCO activity, thereby redirecting the metabolic flux towards secondary metabolism. Similarly, the upregulated expression of the DXS gene, which drives the precursors of the methylerythritol-phosphate (MEP) pathway to their end products such as monoterpenoids: geraniol and linalool, aligns with the metabolic shift from primary to secondary terpenoid biosynthesis. This transformative role of endophytic association highlights the remarkable ability of endophytes to regulate interconnected physiological processes, including improved nitrate uptake, enhanced carbon assimilation, and boosted antioxidant capacity driving significant improvements in growth, development and yield of the host plant.

内生水稻假单胞菌CB24通过单半乳糖二酰基甘油驱动脂质重编程促进光合作用。
由于其精油的价值,天竺葵受到其生理和植物化学的内共生关联的显著影响,尽管驱动这种调节的确切机制在很大程度上仍未被探索。本研究表明,将米氏假单胞菌CB24接种到植物中,显著改变了植物的脂质动力学,导致叶绿体中单半乳糖基二酰基甘油(MGDG)和磺基喹啉基二酰基甘油(SQDG)等脂质积累增加。这是通过增强前体如udp -6-磺基喹诺糖和乙酰辅酶a,以及上调对甘油脂代谢至关重要的plc、MGD1和SQD2等基因来实现的。甘油脂是类囊体膜稳定性所必需的,导致天线蛋白相关基因的显著上调,光收集复合物即LHCA2, LHCA1, PsaA, PetE, PetF, PsbO。P. oryzihabitans还提高了RuBisCO活性,从而将代谢通量转向次级代谢。同样,DXS基因的表达上调,驱动甲基赤四醇-磷酸(MEP)途径的前体到其最终产物,如单萜类:香叶醇和芳樟醇,与从初级到次级萜类生物合成的代谢转变相一致。这种内生关联的转化作用强调了内生菌调节相互关联的生理过程的显着能力,包括改善硝酸盐吸收,增强碳同化,提高抗氧化能力,从而显著改善寄主植物的生长,发育和产量。
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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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