Dysregulation of lncRNA OIP5-AS1 participants in the progression of diabetic retinopathy and affects the proliferation in retinal vascular endothelial cell.
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引用次数: 0
Abstract
Aims: This study was to ascertain the clinical performance of OIP5-AS1 in diabetic retinopathy (DR) and its molecular mechanism in the disease progression.
Materials and methods: Subjects included 85 healthy controls, 79 patients with type 2 diabetes mellitus (T2DM), and 114 T2DM-DR patients. qRT-PCR was conducted to measure the relative abundances of OIP5-AS1 and miR-181a-5p in the research subjects. Receiver operating characteristic (ROC) and logistic regression analyses were employed for the diagnostic capability and risk factor prediction. Cell activities were assessed using CCK-8 and transwell assays. Luciferase reporter assay was used for the correlation confirmation of OIP5-AS1 and miR-181a-5p. Bioinformatic analysis was applied to predict the potential targets of miR-181a-5p.
Results: A significant decrease of OIP5-AS1 was detected in serum from patients with T2MD and T2DM-DR (P < 0.001), exhibiting a high diagnostic value for detecting T2DM (AUC = 0.973) and T2DM-DR patients (AUC = 0.913). OIP5-AS1 was an independent protective indicator for the onset of T2DM-proliferative DR (T2DM-PDR; P = 0.021, OR = 0.306, 95%CI = 0.112-0.837). OIP5-AS1 was markedly reduced in human retinal vascular endothelial cells (HRVECs) with high glucose (HG) (P < 0.001). Overexpression of OIP5-AS1 could significantly suppress the cell growth of HRVECs (P < 0.001). OIP5-AS1 was negatively correlated with miR-181a-5p (r = -0.5327, P < 0.001). Additionally, the impacts caused by OIP5-AS1 on cell events were canceled by transfection of miR-181a-5p mimic (P < 0.001). The possible targets of miR-181a-5p were mined, suggesting mainly enriched in cellular senescence and the MAPK signaling pathway.
Conclusions: OIP5-AS1 was downregulated in T2DM-DR patients and regulated cellular functions via targeting miR-181a-5p. It might offer a new therapeutic target for the disease.
期刊介绍：
Journal of Diabetes Investigation is your core diabetes journal from Asia; the official journal of the Asian Association for the Study of Diabetes (AASD). The journal publishes original research, country reports, commentaries, reviews, mini-reviews, case reports, letters, as well as editorials and news. Embracing clinical and experimental research in diabetes and related areas, the Journal of Diabetes Investigation includes aspects of prevention, treatment, as well as molecular aspects and pathophysiology. Translational research focused on the exchange of ideas between clinicians and researchers is also welcome. Journal of Diabetes Investigation is indexed by Science Citation Index Expanded (SCIE).
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